Transcriptome temporal and functional analysis of liver regeneration termination

Title:

Transcriptome temporal and functional analysis of liver regeneration termination

Creator:

Rychtrmoc, D., Hubálková, L., Víšková, A., Antonín Libra, Martin Bunček, and Zuzana Červinková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:1ed20c69-3f2b-47d8-aad6-2d9a3bb385d3
uuid:1ed20c69-3f2b-47d8-aad6-2d9a3bb385d3

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, liver regeneration, hepatectomy, gene expression profiling, termination, rat, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Decades of liver regeneration studies still left the termination phase least elucidated. However regeneration ending mechanisms are clinicaly relevant. We aimed to analyse the timing and transcriptional control of the latest phase of liver regeneration, both controversial. Male Wistar rats were subjected to 2/3 partial hepatectomy with recovery lasting from 1 to 14 days. Time-series microarray data were assessed by innovative combination of hierarchical clustering and principal component analysis and validated by real-time RT-PCR. Hierarchical clustering and principal component analysis in agreement distinguished three temporal phases of liver regeneration. We found 359 genes specifically altered during late phase regeneration. Gene enrichment analysis and manual review of microarray data suggested five pathways worth further study: PPAR signalling pathway; lipid metabolism; complement, coagulation and fibrinolytic cascades; ECM remodelling and xenobiotic biotransformation. Microarray findings pertinent for termination phase were substantiated by real-time RT-PCR. In conclusion, transcriptional profiling mapped late phase of liver regeneration beyond 5th day of recovery and revealed 5 pathways specifically acting at this time. Inclusion of longer post-surgery intervals brought improved coverage of regeneration time dynamics and is advisable for further works. Investigation into the workings of suggested pathways might prove helpful in preventing and managing liver tumours., D. Rychtrmoc, ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:Suppl 2

Harvested from:

CDK

Metadata only:

false