Lectin-like sequences in genome of Borrelia burgdorferi

Title:

Lectin-like sequences in genome of Borrelia burgdorferi

Creator:

Rudenko, Natalia, Golovchenko, Maryna, and Grubhoffer, Libor

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:04c58271-2c8f-45fd-bf8e-e286500e16f3
uuid:04c58271-2c8f-45fd-bf8e-e286500e16f3
urnnbn:urn:nbn:cz:aba007-000dr3

Subject:

Borrelia burgdorferi, hemagglutination, genomic library, recombinant, and lectin

Type:

model:internalpart and TEXT

Format:

print

Description:

Using degenerative primers designed on the basis of known sequences of lectin genes from different sources a fragment of genomic DNA of Borrelia burgdorferi ( strain B31) that contained a lectin-like sequence was isolated, cloned and sequenced. The presence of an open reading frame of 268 amino acids (position 1501-2304 bp) and the computer analysis of the predicted amino acid sequence showed 37% of identity and 75% of homology over region of 25 amino acids with the legume lectin proteins, including erythroagglutinating phytohcmagglutinin (РНЛ-Е) and leucoagglutinating phylohemagglutinin (PHA-L). The further analysis of the predicted amino acid sequence showed the presence of another two domains (positions 198-211 and 215-226 aa) consisting of the characteristic conserved sequence motifs for legume lectin proteins. Hemagglutinating activity was detected in lysate of В burgdorferi (strain B31) spirochete and the affinity to fetuin was determined in a hemagglutination inhibition test. Hemagglutinating activity was also found in a crude lysate of the recombinant clones carrying the fragment of B. burgdorferi genomic DNA. The inhibition of agglutinating activity by fetuin, D-galactosamine and D-mannosamine was determined using the standard procedure of hemagglutination inhibition test with native rabbit red blood cells (RBC).

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

81-90 and 3-12

Source:

Folia parasitologica | 1999 Volume:46 | Number:2

Harvested from:

CDK

Metadata only:

false