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Start Over Creator Golovchenko, Maryna

3. Detection of Anaplasma DNA in Ixodes ricinus ticks: pitfalls

Creator:
Šikutová, Silvie, Rudolf, Ivo, Golovchenko, Maryna, Rudenko, Nataliia, Grubhoffer, Libor, and Hubálek, Zdeněk
Format:
bez média and svazek
Type:
model:article and TEXT
Language:
English
Description:
A total of 150 nymphal Ixodes ricinus (L., 1758) (Acari: Ixodidae) from the Czech Republic were examined for Anaplasma phagocytophilum (Foggie, 1949) Dumler et al., 2001 by PCR using EHR521/747 primers: 22 of 50 pools were positive (minimum prevalence, 14.7%). However, sequencing of the PCR products did not show complete homology with A. phagocytophilum (91%) while the closest relationship (95%) was found to "Candidatus Ehrlichia walkerii". The results indicate a need for care in interpretation of Anaplasma PCR results and for PCR optimization for detecting A. phagocytophilum in ticks.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Lectin-like sequences in genome of Borrelia burgdorferi

Creator:
Rudenko, Natalia, Golovchenko, Maryna, and Grubhoffer, Libor
Format:
print
Type:
model:internalpart and TEXT
Subject:
Borrelia burgdorferi, hemagglutination, genomic library, recombinant, and lectin
Language:
English
Description:
Using degenerative primers designed on the basis of known sequences of lectin genes from different sources a fragment of genomic DNA of Borrelia burgdorferi ( strain B31) that contained a lectin-like sequence was isolated, cloned and sequenced. The presence of an open reading frame of 268 amino acids (position 1501-2304 bp) and the computer analysis of the predicted amino acid sequence showed 37% of identity and 75% of homology over region of 25 amino acids with the legume lectin proteins, including erythroagglutinating phytohcmagglutinin (РНЛ-Е) and leucoagglutinating phylohemagglutinin (PHA-L). The further analysis of the predicted amino acid sequence showed the presence of another two domains (positions 198-211 and 215-226 aa) consisting of the characteristic conserved sequence motifs for legume lectin proteins. Hemagglutinating activity was detected in lysate of В burgdorferi (strain B31) spirochete and the affinity to fetuin was determined in a hemagglutination inhibition test. Hemagglutinating activity was also found in a crude lysate of the recombinant clones carrying the fragment of B. burgdorferi genomic DNA. The inhibition of agglutinating activity by fetuin, D-galactosamine and D-mannosamine was determined using the standard procedure of hemagglutination inhibition test with native rabbit red blood cells (RBC).
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. Lyme borreliosis: insights into tick- / host-borrelia relations

Creator:
Grubhoffer, Libor, Golovchenko, Maryna, Vancová, Marie, Zacharovová-Slavíčková, Klára, Rudenko, Nataliia, and Oliver Jr., James H.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
borrelia spirochete, Lyme borreliosis, ticks, vectors, interactions, and glycoproteins
Language:
English
Description:
Lyme borreliosis (LB) is a serious infectious disease of humans and some domestic animals in temperate regions of the Northern Hemisphere. It is caused by certain spirochetes in the Borrelia burgdorferi sensu lato (s.l.) species complex. The complex consists of 11 species (genospecies). Borrelia burgdorferi sensu stricto (s.s.), Borrelia garinii and Borrelia afzelii are the major agents of human disease. Borrelia burgdorferi s.l. species are transmitted mainly by ticks belonging to the Ixodes ricinus species complex plus a few additional species not currently assigned to the complex. B. burgdorferi infections may produce an acute or chronic disease with a wide array of clinical symptoms such as erythema migrans (EM), carditis, arthritis, neuroborreliosis, and acrodermatitis chronica atrophicans (ACA). Differences in LB spirochetes ''genospecies'' and strains/isolates determine the occurrence and severity of this multi-system disease. Accurate and reliable identification of the LB spirochetes in ticks as well as knowledge of their prevalence are essential for prevention against the disease and development of an effective vaccine. An overview of the knowledge of molecular factors with emphasis on potential protein-carbohydrate interactions in the tick-borrelia system is the main focus of this review.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public