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2. Hepcidin expression in adipose tissue increases during cardiac surgery

Creator:
Martin Vokurka, Zdeňka Lacinová, Jaromír Křemen, Kopecký, P., Jan Bláha, Květa Pelinková, Haluzík, M., and Emanuel Nečas
Format:
print
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, železo, záněty, chirurgie, iron, inflammations, surgery, hepcidin, adipose tissue, 14, and 612
Language:
English
Description:
Hepcidin, a key regulator of iron metabolism, plays a crucial role in the pathogenesis of anemia of chronic disease. Although it is produced mainly in the liver, its recently described expression in adipose tissue has been shown to be enhanced in massive obesity due to chronic low-grade inflammation. Our objective was to study the changes in hepcidin expression in adipose tissue during acute-phase reaction. We measured hepcidin mRNA expression from isolated subcutaneous and epicardial adipose tissue at the beginning and at the end of the surgery. The expression of mRNAs for hepcidin and other iron-related genes (transferrin receptor 1, divalent metal transporter 1, ferritin, ferroportin) were measured by real-time RT-PCR. Hepcidin expression significantly increased at the end of the surgery in subcutaneous but not in epicardial adipose tissue. Apart from the increased levels of cytokines, the parameters of iron metabolism showed typical inflammation-induced changes. We suggest that acute inflammatory changes could affect the regulation of hepcidin expression in subcutaneous adipose tissue and thus possibly contribute to inflammation-induced systemic changes of iron metabolism., M. Vokurka ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Plasma prohepcidin as a negative acute phase reactant after large cardiac surgery with a deep hypothermic circulatory arrest

Creator:
Pavel Maruna, Jaroslav Lindner, Kunštýř, J., Kateřina M. Plocová, and Jaromír Hubáček
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, fyziologie, proteiny akutní fáze, cytokiny, chirurgie, physiology, acute phase proteins, cytokines, surgery, interleukin-6, 14, and 612
Language:
English
Description:
Hepcidin is a key regulator of iron metabolism and a mediator of anemia in inflammation. Recent in vitro studies recognized prohepcidin as a type II acute phase protein regulating via interleukin-6. The aim of the present study was to investigate the time course of plasma prohepcidin after a large cardiac surgery in relation to IL-6 and other inflam matory parameters. Patients with chronic thromboembolic hypertension (n=22, males/females 14/8, age 51.9±10.2 years) underwent pulmonary endarterectomy using cardiopulmonary bypass and deep hypothermic circulatory arrest were included into study. Arterial concentrations of prohepcidin, IL-1β , IL-6, IL-8, tumor necrosis factor-α , and C-reactive protein were measured before/after sternotomy, after circulatory arrest, after separation from bypass, and then 12, 18, 24, 36, 48 h and 72 h after the separation from bypass. Hemodynamic parameters, hematocrit and markers of iron metabolism were followed up. Pulmonary endarterectomy induced a 48 % fall in plasma prohepcidin; minimal concentrations were detected after separation from cardiopulmonary bypass. Prohepcidin decline correlated with an extracorporeal circulation time (p<0.01), while elevated IL-6 levels were inversely associated with duration of prohepcidin decline. Postoperative prohepcidin did not correlate with markers of iron metabolism or hemoglobin concentrations within a 72-h period after separation from CPB. Prohepcidin showed itself as a negative acute phase reactant during systemic inflammatory response syndrome associated with a cardiac surgery. Results indicate that the evolution of prohepcidin in postoperative period implies the antagonism of stimulatory effect of IL-6 and contraregulatory factors inhibiting prohepcidin synthesis or increasing prohepcidin clearance., P. Maruna ... [et al.]., and Obsahuje seznam literatury
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Prediction of adrenocortical insufficiency after pituitary adenoma surgery using postoperative basal cortisol levels

Creator:
Hána, V., Jana Ježková, Mikuláš Kosák, Michal Kršek, Marek, J., David Netuka, Hill, M., and Václav Hána
Format:
print, bez média, and svazek
Type:
article, články, model:article, and TEXT
Subject:
Fyziologie člověka a srovnávací fyziologie, adenomy hypofýzy, chirurgie, pituitary adenomas, surgery, adrenal insufficiency, hypocorticalism, ACTH test, 14, and 612
Language:
English
Description:
Our aim was to analyze the correlation of early postoperative cortisol levels in patients after transsphenoidal pituitary adenoma surgery compared to the standard dose ACTH test and Insulin tolerance test (ITT) several months later. We retrospectively reviewed data from 94 patients operated for pituitary adenoma in years 2009-2012. The comparison of day 7 (median) postoperative basal cortisol levels and 3.6 months (median) after pituitary adenoma surgery stimulation test - standard dose 250 μg 1-24ACTH test in 83 patients or ITT in 11 patients were performed. All 16 patients with early postoperative cortisol levels >500 nmol/l proved a sufficient response in the stimulation tests. At basal cortisol levels of 370-500 nmol/l the sufficient response was found in 96 % (27/28) of patients. In the postoperative basal cortisol levels 200-370 nmol/l we found a preserved corticotroph axis later on in 88 % (28/32) of cases. Patients with basal cortisol levels 100-200 nmol/l had a maintained corticotroph axis function in 8/11 cases - 73 %. All patients with an early postoperative basal cortisol level above 500 nmol/l proved in the stimulation tests a preserved corticotroph axis function. The interval 370-500 nmol/l showed a minimal risk of postoperative adrenal insufficiency., V. Hána Jr., J. Ježková, M. Kosák, M. Kršek, J. Marek, D. Netuka, M. Hil, V. Hána., and Obsahuje bibliografii
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

5. Z pamětí prof. MUDr. Emericha Poláka, DrSc.

Creator:
Hlaváčková, Ludmila and Mášová, Hana
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Prof. Emerich Polák, history of medicine, interwar Czechoslovakia, surgery, and health institutions
Language:
Czech
Description:
Profesor Emerich Polák (1901-1980) byl ve své době jedním z našich nejvýznamnějších chirurgů. Byl nejen vyhlášeným operatérem ovládajícím chirurgii v celé její šíři, ale i autorem řady významných publikací monografických a časopiseckých, autorem a spoluautorem učebnic svého oboru. V polovině 70. let 20. století začal psát své paměti a počítal s jejich vydáním, k čemuž ovšem nedošlo. Paměti obsahují mnoho informací nejen o vývoji chirurgie ve 20. století očima odborníka, ale i o kulturním a společenském životě nebo o těžkých letech okupace. Text pamětí o rozsahu 428 stran se uchoval v několika strojopisech. Vzhledem k tomu, že v dohledné době není naděje na jejich vydání vcelku, budou publikovány alespoň některé jejich části časopisecky. Vybraly jsme část věnovanou popisu každodennosti v podolském sanatoriu a životu ve státní nemocnici v Mukačevě. V podolském sanatoriu Polák působil od roku 1925 do konce roku 1934. Od roku 1921 rovněž jako asistent II. kliniky, po deset let odjížděl operovat do nemocnice v Mukačevě. Část věnovanou sanatoriu v Podolí edičně připravila Ludmila Hlaváčková, část zachycující vzpomínky na Mukačevo připravila Hana Mášová., Professor Emerich Polák (27. 5. 1901 - 27. 8. 1980) was one of the most prominent Czechoslovak surgeons. In 1970s he started to write his memoirs and prepared them for publication, but they stayed in manuscript. The text of 428 pages was preserved in a few copies. It includes a lot of information about development of the surgery in the first half of the 20th century, and much about cultural and social life of that time. The two parts, that were chosen for publication in this magazine, describe the years Emerich Polák spent at the 2nd surgery clinic in the sanatorium in Podolí (Prague) and his ten years in the hospital in Mukačevo, where he worked in the summer seasons since 1921. (Translated by Hana Mášová.), and Překlad resumé: Hana Mášová
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public