In the subcontinental, semiarid lowland region of Central Bohemia (Czech Republic), continuous human impact acting together with diverse natural environmental conditions resulted in the present extraordinarily complex pattern of vegetation. Three radiocarbon-dated pollen diagrams for the area indicate that this complexity results from past vegetation development. During prehistory, places suitable for settlement (with respect to climate, geology, hydrology, etc.) were colonized and transformed first. This resulted in a diachrony in vegetation development due to human activity starting in the first half of the Holocene. This caused an increase in diversity in the region as plant species persisting from previous periods, along with those associated with different agricultural practices, increased. Local abiotic factors affected not only the chronology of human impact but also its specific effects on the ecosystem. Anthropogenic pressure may have had different effects under different conditions. Human population pressure was the mediator between the abiotic diversity and selectively transformed vegetation suitable for the respective habitats. Differences in the chronology of human impact, mixed oak woodland degradation, and the chronology of beech, silver fir and hornbeam expansion are documented for the different ecological zones of the study area. These differences shed light on the mechanisms resulting in some of the important changes in Holocene vegetation. In the absence of man, the decline in mixed oak woodlands, typical of the Middle Holocene in Central Bohemia, would have been probably much slower and less extensive. Unlike in the uplands and mountains, the expansion in the area of beech, silver fir and hornbeam would have been insignificant. The present vegetation resulted to a large extent from management during High Middle Ages. There is almost no continuity in vegetation from the late prehistory to the present.