Both, common gene variants and human adenovirus 36 (Adv36) are involved in the pathogenesis of obesity. The potential relationship between these two pathogenic factors has not yet been investigated. The aim of our study was to examine the association of obesity susceptibility loci with Adv36 status. Genotyping of ten gene variants (in/near TMEM18, SH2B1, KCTD15, PCSK1, BDNF, SEC16B, MC4R, FTO) and analysis of Adv36 antibodies was performed in 1,027 Czech adolescents aged 13.0-17.9 years. Variants of two genes (PCSK1 and BDNF) were associated with Adv36 seropositivity. A higher prevalence of Adv36 antibody positivity was observed in obesity risk allele carriers of PCSK1 rs6232, rs6235 and BDNF rs4923461 vs. noncarriers (χ2=6.59, p=0.010; χ2=7.56, p=0.023 and χ2=6.84, p=0.033, respectively). The increased risk of Adv36 positivity was also found in PCSK1 variants: rs6232 (OR=1.67, 95 % CI 1.11-2.49, p=0.016) and rs6235 (OR=1.34, 95 % CI 1.08-1.67, p=0.010). PCSK1 rs6232 and BDNF rs925946 variants were closely associated with Adv36 status in boys and girls, respectively (χ2=5.09, p=0.024; χ2=7.29, p=0.026). Furthermore, PCSK1 rs6235 risk allele was related to Adv36 seropositivity (χ2=6.85, p=0.033) in overweight/obese subgroup. In conclusion, our results suggest that obesity risk variants of PCSK1 and BDNF genes may be related to Adv36 infection., L. Dušátková, H. Zamrazilová, I. Aldhoon Hainerová, R. L. Atkinson, B. Sedláčková, Z. P. Lee, J. Včelák, B. Bendlová, M. Kunešová, V. Hainer., and Obsahuje bibliografii
Obesity in childhood increases the risk of obesity in adulthood and is predictive for the development of metabolic disorders. The fatty acid composition is associated with obesity and obesityassociated disorders. We investigated the relationship between serum fatty acids composition, adiposity, lipids profile, parameters of glucose metabolism and leptin. The study subjects were 380 adolescents aged 15.0-17.9 years. The study's variables included anthropometric measurements, levels of serum lipids and hormonal parameters. Individual fatty acids were determined in plasma by gas-liquid chromatography. Palmitoleic acid (16:1n-7, PA) significantly positively correlated with percentage of body fat. Saturated fatty acids in phospholipids (PL) positively correlated with BMI and percentage of body fat. PA content in all lipids classes positively correlated with total cholesterol (TC), HDL cholesterol, triglycerides (TG) levels. Stearoyl-CoA desaturase (SCD) activity positively correlated with percentage of body fat and positive correlations of SCD and PA level with leptin were found. Plasma PA content and SCD are associated with adiposity and leptin in obese adolescents. No significant correlation between PA level and insulin resistance was found. Palmitoleate positively correlated with TC, HDL cholesterol, TG and LDL cholesterol levels., P. Hlavaty, E. Tvrzicka, B. Stankova, H. Zamrazilova, B. Sedlackova, L. Dusatkova, V. Hainer, M. Kunesova., and Obsahuje bibliografii
The aim of the study was to translate the Intrapersonal Curiosity Scale (InC) into the Czech language and to verify its factor structure and psychometric properties on the sample of grammar school students in the Czech Republic. The Intrapersonal Curiosity Scale InC is a 12-item tool that measures the tendency to inquisitively explore own identity and inner experience in order to better understand own inner self. The sample consisted of 1020 participants, of which 353 (34.6%) were boys, 665 (65.2%) were girls, and two participants (0.2%) did not state their sex or age. The authors compared several considered models using CFA. The results suggest that the original theoretical model, which calculates the total InC score as the sum of three mutually correlated subscales EPI, ROP and UEM, is still uncertain in the Czech version verified on a group of adolescents. In the authorsʼ opinion, the version with three factors with minor corrections fits to the data best of all compared models, although with a lack of evidence for the unidimensionality of the overall InC scale. In this model, verification of the weak invariance of the measurements was achieved when comparing girls and boys using MGCFA. Significant correlations of latent scores of EPI, ROP and UEM subscales with other assessed constructs were found., Cílem studie bylo adaptovat Škálu intrapersonální zvídavosti (Intrapersonal Curiosity, InC) do českého jazyka a ověřit její faktorovou struk-turu a psychometrické vlastnosti na souboru studentů gymnázií v České republice. Škála intrapersonální zvídavosti InC je 12položkovým nástrojem, který zachycuje tendenci zvídavě zkoumat svoji identitu a vlastní prožívání s cílem lépe porozumět vlastnímu nitru. Výběrový soubor tvořilo celkem 1020 participantů, z toho bylo 353 chlapců (34,6 %), 665 dívek (65,2 %) a dva participanti (0,2 %) svůj gender ani věk neuvedli. V rámci analýz autoři pomocí CFA porovnávali několik uvažovaných modelů. Výsledky naznačují, že původní teoretický model, který počítá celkový skór InC jakou součet tří vzájemně korelovaných subškál EPI, ROP a UEM, je u české verze ověřované na souboru adolescentů nejistý. Verze se třemi faktory dle názoru autorů odpovídá s drobnými korekcemi datům nejlépe ze všech porovnávaných modelů, byť s nedostatkem důkazů pro unidimenzionalitu celkové škály InC. Na tomto modelu bylo dosaženo ověření slabé invariance měření při porovnávání dívek a chlapců pomocí MGCFA. Byly nalezeny významné korelace latentních skórů subškál EPI, ROP a UEM s ostatními posuzovanými konstrukty., Martina Friedlová, Zuzana Vaculčíková Sedláčková, Miroslav Charvát., and Obsahuje bibliografické odkazy