The New World grasshopper Cornops aquaticum (Leptysminae: Acrididae) shows a geographical pattern for three Robertsonian polymorphisms in its southernmost area of distribution in Argentina and Uruguay. The frequency and distribution of chiasmata were analysed in five Argentinian populations. This study reveals a strong redistribution of chiasmata in fusion carriers, with a reduction in proximal and increase of distal chiasma frequency in fusion bivalents and trivalents, when all three karyotypes were compared. However, when only fusion bivalents and trivalents were compared, chiasma frequency was significantly higher in the former than in the latter. This higher chiasma frequency in fusion bivalents is due to an increase in proximal chiasma frequency. It is argued that the reduction in proximal chiasma frequency (relative to unfused bivalents) in fusion bivalents may be due to interference across the centromere. Proximal chiasma reduction in trivalents may be attributed either to a physical effect of structural heterozygosity or to an adaptation to the polymorphic condition. Therefore the differences in the distribution of chiasmata in trivalents and Robertsonian bivalents have different causes.