Thrips are important agricultural pests and accurate identification is important for their effective management. In order to determine species, however, they need to be mounted on slides and the traditional process is time-consuming. The aim of this paper is to describe a simple and fast method to prepare temporary slides for the routine identification of thrips, which is not dependent on their colour and hardness. Four species of thrips of different colours were used in the preparations: Frankliniella occidentalis (yellow with brown tergal markings), Frankliniella schultzei (entirely brown), Haplothrips gowdeyii (dark brown to carmine) and Caliothrips phaseoli (brown to black). Slides of each species were prepared using three different methods: traditional (3 days), simplified (6 h) and fast method (10 min). The thrips on the resulting slides were observed under a microscope and important structures used in their identification were compared. The quality of the slides prepared using the traditional method was superior to those prepared using the other two methods if only the transparency and general position of the insects on the slides were considered. The transparency of the slides prepared using the simplified method was also good, but only for the pale coloured species (yellow and grey-brown). The fast method, on the other hand, was very efficient for routine identification since it resulted in slides of sufficient quality for identifying species regardless of their colour. It is important, however, to stress that the fast method is only suitable for preparing temporary slides for routine identification and is not a substitute for the traditional method of preparing permanent slides., Luís C.P. Silveira, Marcelo M. Haro., and Obsahuje bibliografii
Integrated Population Modelling (IPMs) is a computational method for estimating population and demographic parameters that can improve precision relative to traditional methods. Here we compare the precision of IPM to traditional mark-recapture analysis to estimate population parameters in the common dormouse (Muscardinus avellanarius). This species is relatively rare across its European range and field estimation of demographic parameters can be challenging, as several parts of the life history are difficult to observe in the field. We develop an IPM model incorporating dormouse nest counts and offspring counts, which is data often recorded as a standard part of dormouse nest box monitoring. We found a significant improvement in precision in the estimation of demographic parameters using IPM compared to standard mark-recapture estimation. We discuss our results in the context of common dormouse conservation monitoring.