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2. Environmentální rastrovací elektronová mikroskopie a její aplikační možnosti
- Creator:
- Neděla, Vilém, Roubalíková, Lenka, and Weyda, František
- Format:
- bez média and svazek
- Type:
- model:article and TEXT
- Subject:
- environmental scanning electron microscopy, AQUASEM II, amber, tissue of fossil insect, morphological analysis of root canal walls, environmentální rastrovací elektronová mikroskopie, jantar, tkáně fosilního hmyzu, and morfologie stěny kořenových kanálků zubů
- Language:
- Czech
- Description:
- Article is focused on environmental scanning electron microscopy as a universal method for investigation of insulating and water containing samples. Advantages of this microscopy method are demonstrated by experiments that study biological tissues of fossil insects embedded in amber or analyze morphological structure of root canal walls prepared by laser. and Článek se zabývá environmentální rastrovací elektronovou mikroskopií jako univerzální metodou pro zkoumání izolačních vzorků a vzorků obsahujících vodu. Výhody této mikroskopické metody jsou presentovány na základě experimentů zabývajících se studiem zbytků biologických tkání fosilního hmyzu v jantaru a studiem povrchu stěny kořenových kanálků zubů po preparaci pevnolátkovým laserem.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. Kryptobiotické stavy: aneb Vratná zastavení životních dějů. 1. Když přijdou sucha - anhydrobióza
- Creator:
- Košťál, Vladimír and Weyda, František
- Type:
- article, model:article, and TEXT
- Language:
- Czech
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
4. Mikroradiografie biologických objektů pixelovými detektory
- Creator:
- Dammer, Jiří and Weyda, František
- Type:
- article and TEXT
- Language:
- Czech
- Description:
- Techniky zobrazování rentgenovým zářením prodělaly za poslední období velký vývoj, kdy jsou tradiční systémy nahrazovány digitálními technologiemi, zejména založenými na rentgenech s mikroohniskem a digitálních pixelových detektorech. Autoři popisují jejich praktické využití při studiu biologických objektů. and X-ray radiation examination, projection and screening techniques have recently seen dramatic developments, with traditional approaches replaced by digital technologies, particularly those based on microfocus X-ray devices and digital pixel detectors. The authors present their practical use in various biological studies.
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/
5. Ultrastructural study of tergal and posterior sternal glands in Prorhinotermes simplex (Isoptera: Rhinotermitidae)
- Creator:
- Šobotnik, Jan, Weyda, František, and Hanus, Robert
- Type:
- article, model:article, and TEXT
- Subject:
- Ultrastructure, tergal gland, posterior sternal gland, secretory cell class 2, termite, Isoptera, Rhinotermitidae, Prorhinotermes simplex, and imagoes
- Language:
- English
- Description:
- In Prorhinotermes simplex, tergal glands are present on the last three tergites (from the 8th to the 10th) in imagoes of both sexes. In addition, males possess posterior sternal glands of the same structure on sternites 8 and 9. The tergal and the posterior sternal glands consist of four cell types: class 1 and class 2 secretory cells, and class 3 cells with corresponding canal cells. The cytoplasm of class 1 cells contains smooth endoplasmic reticulum, elongated mitochondria and numerous microtubules. Apical parts of these cells are formed by dense and long microvilli with a central ductule. Class 2 cells contain predominantly lucent vacuoles (in females) or lipid droplets (in males). The structure of class 3 cells does not differ from class 3 cells found in other body parts.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
6. Ultrastructure of the frontal gland in Prorhinotermes simplex (Isoptera: Rhinotermitidae) and quantity of the defensive substance
- Creator:
- Šobotník, Jan, Weyda, František, Hanus, Robert, Kyjaková, Pavlína, and Doubský, Jan
- Type:
- article, model:article, and TEXT
- Subject:
- Ultrastructure, frontal gland, termite, Isoptera, Rhinotermitidae, Prorhinotermes simplex, Prorhinotermes inopinatus, intercaste, defence secretion, and E-1-nitropentadecene
- Language:
- English
- Description:
- The frontal gland as a sac-like organ in Prorhinotermes simplex is present only in presoldiers, soldiers, and imagoes, but exists also in nymph-soldier intercastes. The secretory epithelium consists of a single type of secretory cells adhering directly to the cuticular intima. Secretory vacuoles originate in electron dense vesicles, which are transformed into large electron lucent vacuoles. Intermediate vacuoles frequently contain lipid droplets. The frontal gland cells in presoldiers reveal modifications connected with the production of a new cuticle; the new cuticle is thin and compact, whereas the old one is thick, porous, and wrinkled. None of these cuticles is present in soldiers (sic!). In soldiers, the cuticular intima is of endocuticular origin and is formed by dispersed dense material; the apical parts of secretory cells are formed by numerous irregular finger-like projections, true microvilli are completely lacking. In imagoes, the cuticle is composed of an epicuticle, a layer of epicuticular filaments, and one more basal layer; sexual differences were not observed. In nymph-soldier intercastes, the structure of the gland differs in the head and in the metathorax; the head part of the gland resembles the imaginal gland whereas the thoracic part resembles more that of the soldier; the development of secretory vacuoles stops at the stage of presence of lipid droplets. E-1-nitropentadecene was found in the highest amount in soldiers (comparable to P. inopinatus soldiers), in moderate amount in imagoes, and it is missing in both presoldiers and intercastes.
- Rights:
- http://creativecommons.org/publicdomain/mark/1.0/ and policy:public
7. Vysokorychlostní digitální fotografie v biologii
- Creator:
- Weyda, František
- Type:
- article and TEXT
- Language:
- Czech
- Description:
- Rychlé až velmi rychlé procesy na úrovni organismů nebo tkání jsou v biologii velmi časté. Kamery specializované na vysokorychlostní video jsou velmi drahé. Některé současné a relativně levné digitální fotoaparáty dokáží pořizovat videosekvence při vysoké rychlosti (zatímco 30 snímků za sekundu je standardní rychlost, tyto moderní fotoaparáty dokáží snímat až 1200 snímků za sekundu). and Rapid and very rapid processes at the level of the entire organism as well as at the tissue level are very common in biology. Some recent and relatively cheap digital cameras enable biologists to shoot videosequences at a high rate (while 30 frames per second is the standard rate, such modern cameras go up to 1 200 fps).
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/