Mesembryanthemum crystallinum is an annual succulent plant that is being used as an emerging healthy leafy vegetable. To investigate the growth and physiological response of M. crystallinum to artificial lighting, five different light treatments were applied at 150 µmol(photon) m-2 s-1, which were white (W), different rations of red/blue (B) (15, 40, and 70%B), and blue (100%B), respectively. Our results showed that plants could gain as much as edible leaf area and dry mass with a certain ratio of blue (40%) in comparison with W. Plants grown under 100%B resulted in reduced photosynthetic rate, leaf area, and fresh mass compared with W. Adding blue fraction in the light regime enhanced the photosynthetic performance by influencing the amount of chlorophyll (Chl), Chl a/b, and specific leaf area. Under red/blue treatments, the electron transport rate and effective quantum yield of both PSII and PSI increased, while the nitrate content was reduced and flavonoids and total antioxidant capacity were unaffected.