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Start Over Creator Smetana, K.

1. Dominant nucleolus in the progenitor cell using human bone marrow erythroid and granulocytic cell lineages as a model. A morphological and cytochemical note

Creator:
Smetana, K., Klamová, H., and Mikulenková, D.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
dominant nucleoli and progenitor cells
Language:
English
Description:
Progenitor cells of the human erythroid and granulocytic cell lineages are characterized by the presence of several nucleoli. One of these nucleoli is larger and possesses more fibrillar centres than others. Such nucleolus is apparently dominant in respect of both size and main nucleolar function such as nucleolar-ribosomal RNA transcription. Such nucleolus is also visible in specimens using conventional visualization procedures, in contrast to smaller nucleoli. In the terminal differentiation nucleated stages of the erythroid and granulocytic development, dominant nucleoli apparently disappeared, since these cells mostly contained very small nucleoli of a similar size with one fibrillar centre. Thus, the easily visible dominant nucleoli appear to be useful markers of the progenitor cell state, such as proliferation, and differentiation potential.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

2. Genistein induces Bcl-2 expression in human dermal microvascular endothelial cells: a short report

Creator:
Lachová, V., Mitrengová, P., Melegová, N., Smetana, K., and Gál, P.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
wound healing, angiogenesis, hormone replacement therapy, and phytoestrogen
Language:
English
Description:
It has been shown previously that oestradiol protects the vascular network, leading to increased skin flap viability associated with Bcl-2, VEGF and FGF-2 up-regulation. We have shown that genistein, a natural selective oestrogen receptor modulator, also increases skin flap viability in rats and induces Bcl-2 expression in human umbilical vein endothelial cells. In the present study we aimed to answer the question whether genistein increases expression of Bcl-2, a potent anti-apoptotic protein, in human dermal microvascular endothelial cells (HMVEC-d) as well. Our results showed that administration of genistein induces Bcl-2 expression in a concentration-dependent manner. Cell co-treatment with genistein and anti-ER compounds (MPP, PHTPP, ICI, G-15) diminished the observed positive effect of genistein on Bcl-2 expression. The decrease in Bcl-2 expression in HMVEC-d was most prominent after co-treatment with ICI (nuclear ER antagonist/ GPR30 agonist) and PHTPP (selective ER-β antagonist). In conclusion, genistein increases Bcl-2 expression in HMVEC-d, contributing to its protective effect on the skin flap viability. However, the question whether the mechanism is ER-specific (via ER-β) has to be answered in further studies using a model of gene silencing or genetically modified cells.
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

4. To the nuclear region occupied by nucleolar bodies in human leukaemic myeloblasts of Kasumi 1 and K 562 lineages

Creator:
Smetana, K., Otevřelová, P., Kuželová, K., and Zápotocký , M.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
nucleolar body, nuclear diameter ratio, and cultured human leukaemic granulocytic progenitors
Language:
English
Description:
Previous observation demonstrated that measured nucleolar and nuclear diameters and the resulting calculated ratio might facilitate estimation of the approximate size of the nuclear region occupied by the nucleolar bodies. The size of nuclear regions occupied by nucleolar bodies decreased during the differentiation and maturation of leukaemic lymphocytes, but was constant for each differentiation or maturation stage. The present study was unde-taken to provide more information on the approximate size of the nuclear regions occupied by nucleolar bodies in leukaemic granulocytic progenitors. Myeloblasts of established Kasumi 1 and K 562 cell lineages originating from human myeloid leukaemias were convenient models for such study because they represented only one and early differentiation stage of granulocytic progenitors. According to the results, the maximal and mean nucleolar body : maximal and mean nuclear diameter ratios in myeloblasts without heavy nuclear alterations were stable and not markedly influenced by the anti-leukaemic treatment or aging. Thus, the roughly estimated size of nuclear regions occupied by nucleolar bodies in these cells appeared to be similar and stable regardless of aging or antileukaemic treatment. In contrast, the antileukaemic treatment or aging in such myeloblasts induced marked reduction of the nucleolar biosynthetic activity reflected by the decreased number of nucleolar fibrillar centres. and Corresponding author: Karel Smetana
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public