Search

Start Over Creator Pokorný, Jaroslav

2. Determination of brain water content by dry/wet weight measurement for the detection of experimental brain edema

Creator:
Kozler, Petr, Marešová, Dana, and Pokorný, Jaroslav
Format:
počítač and online zdroj
Type:
model:article and TEXT
Subject:
brain water content, dry/wet weight measurement, cytotoxic edema, and vasogenic edema
Language:
English
Description:
Brain edema is a fatal pathological state in which brain volume increases as a result of abnormal accumulation of fluid within the brain parenchyma. A key attribute of experimentally induced brain edema – increased brain water content (BWC) – needs to be verified. Various methods are used for this purpose: specific gravimetric technique, electron microscopic examination, magnetic resonance imaging (MRI) and dry/wet weight measurement. In this study, the cohort of 40 rats was divided into one control group (CG) and four experimental groups with 8 rats in each group. The procedure for determining BWC using dry/wet weight measurement was initiated 24 h after the completion of edema induction by the water intoxication method (WI group); after the intraperitoneal administration of Methylprednisolone (MP) together with distilled water during edema induction (WI+MP group); 30 min after osmotic blood brain barrier disruption (BBBd group); after injection of MP via the internal carotid artery immediately after BBBd (BBBd + MP group). While induction of brain edema (WI, BBBd) resulted in significantly higher BWC, there was no increase in BWC in the MP groups (WI+MP, BBBd+MP), suggesting a neuroprotective effect of MP in the development of brain edema.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. Effect of methylprednisolone on experimental brain edema in magnetic resonance imaging

Creator:
Kozler, Petr, Herynek, Vít, Marešová, Dana, Perez, Pablo D, Šefc, Luděk, and Pokorný, Jaroslav
Format:
počítač and online zdroj
Type:
model:article and TEXT
Subject:
fyziologie, physiology, methylprednisolone, magnetic resonance imaging, water intoxication, cytotoxic edema, vasogenic edema, 14, and 612
Language:
English
Description:
Magnetic resonance imaging has been used for evaluating of a brain edema in experimental animals to assess cytotoxic and vasogenic edema by the apparent diffusion coefficient (ADC) and T2 imaging. This paper brings information about the effectiveness of methylprednisolone (MP) on experimental brain edema. A total of 24 rats were divided into three groups of 8 animals each. Rats with cytotoxic/intracellular brain edema induced by water intoxication were assigned to the group WI. These rats also served as the additional control group CG when measured before the induction of edema. A third group (WIMP) was intraperitoneally administered with methylprednisolone 100 mg/kg during water intoxication treatment. The group WI+MP was injected with methylprednisolone 50 mg/kg into the carotid artery within two hours after the water intoxication treatment. We evaluated the results in four groups. Two control groups (CG, WI) and two experimental groups (WIMP, WI+MP). Rats were subjected to MR scanning 24 h after edema induction. We observed significantly increased ADC values in group WI in both evaluated areas - cortex and hippocampus, which proved the occurrence of experimental vasogenic edema, while ADC values in groups WIMP and WI+MP were not increased, indicating that the experimental edema was not developed and thus confirming the protective effect of MP., Petr Kozler, Vít Herynek, Dana Marešová, Pablo D. Perez, Luděk Šefc, Jaroslav Pokorný., and Obsahuje bibliografii
Rights:
http://creativecommons.org/publicdomain/mark/1.0/ and policy:public

4. Effect of methylprednisolone on experimental brain edema in rats – own experience reviewed

Creator:
Kozler, Petr, Marešová, Dana, and Pokorný, Jaroslav
Format:
počítač and online zdroj
Type:
model:article and TEXT
Subject:
cytotoxic edema, vasogenic edema, water intoxication, blood brain barrier disruption, and methylprednisolone
Language:
English
Description:
Brain edema – a frequently fatal pathological state in which brain volume increases resulting in intracranial pressure elevation – can result from almost any insult to the brain, including traumatic brain injury. For many years, the objective of experimental studies was to find a method to prevent the development of brain edema at the onset. From this perspective, the use of methylprednisolone (MP) appears promising. High molecular MP (MW>50 kDa) can be incorporated into the brain – in the conditions of the experimental model – either by osmotic bloodbrain barrier disruption (BBBd) or during the induction of cellular edema by water intoxication (WI) – a condition that increases the BBB permeability. The time window for administration of the MP should be at the earliest stages of edema. The neuroprotective effect of MP on the permeability of cytoplasmatic membranes of neuronal populations was proved. MP was administrated in three alternative ways: intraperitoneally during the induction of cytotoxic edema or immediately after finishing cytotoxic edema induction in a dose of 100 mg/kg b.w.; into the internal carotid artery within 2 h after finishing cytotoxic edema induction in a dose of 50 mg/kg b.w.; into internal carotid artery 10 min after edema induction by BBBd in a dose of 50 mg/kg b.w.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public