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2. D-galactose induced changes in enzymatic antioxidant status in rats of different ages
- Creator:
- Hadzi-Petrushev, N., Stojkovski, V., Mitrov, D., and Mladenov, M.
- Format:
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- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, stárnutí, aging, d-galactose, senescence, antioxidant enzymes, lipid peroxidation, wistar rats, 14, and 612
- Language:
- English
- Description:
- Considering the preexisting influence of the process of natural aging on antioxidant enzymes activity and the level of lipid peroxidation, the age of the rats at which D-galactose (D-gal) treatment is started could strongly impact the development of D-gal induced senescence. To eval uate this, we subjected 1, 3 and 15 months old rats to D-gal treatment in parallel with having appropriate placebos (0.9 % saline). Our results showed elevated glutathione peroxidase (GPx) acti vity and no significant changes in superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity or malondialdehyde (MDA) levels in relation to natural aging. In mature and aged senescent livers we observed positive correlation between increased ratio R=SOD/(GPx+CAT) and increased MDA concentration. MDA levels seemed to correlate positively with the age of the animals at which D-gal treatment had started. In the case of 3 and 15 months old rats there was D-gal induced decrease in SOD and GR activity, but this effect of the treatment was not observed in 1 month old rats. Our results imply that the changes in the antioxidant enzyme activities are not only under the influence of the D-gal overload, but also depend on the developmental stage of the rats. According to our resu lts, with regard to enzymatic antioxidant capacity and the level of lipid peroxidation, the best age for induction of senescence is somewhere after the third month., N. Hadzi-Petrushev, V. Stojkovski, D. Mitrov, M. Mladenov., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public
3. The relationship between antioxidant enzymes and lipid peroxidation in senescent rat erythrocytes
- Creator:
- Mladenov, M., Gokik, M., Hadzi-Petrushev, N., Gjorgoski, I., and Jankulovski, N.
- Format:
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- Type:
- article, články, model:article, and TEXT
- Subject:
- Fyziologie člověka a srovnávací fyziologie, kyselina močová, uric acid, D-galactose, antioxidant enzymes, melondialdehyde, rats, 14, and 612
- Language:
- English
- Description:
- The aim of this study was to gain more complete information about the relationships between some endogenous antioxidants and the malondialdehyde (MDA) as a marker of lipid peroxidati on, during D -galactose induced senescence. The activities of superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) and the concentrations of uric acid (UA) in plasma and MDA in erythrocyte’s hemolysate, were determined in 15 D -galactose (D-gal), treated rats and compared with 15 placebo. The activity of the erythrocyte’s CAT was found significantly increased due to the senescence. The ratio of the activities of antioxidant enzymes R =SOD/(GPx+CAT) was significantly decreased due to the sen escence and negatively correlated with the MDA ( ρ = -0.524, p=0.045). The antioxidant enzymes SOD and GPx negatively correlated with the MDA, while CAT displayed no correlation. Further, the UA positively correlated with the ratio of activities of the antioxidant enzymes R=SOD/(GPx+CAT), ( ρ =0.564, p=0.029 for senescent rats). Obtained results may contribute t o better understanding of the process of D-gal induced senescence in the erythrocytes., M. Mladenov, M. Gokik, N. Hadzi-Petrushev, I. Gjorgoski, N. Jankulovski., and Obsahuje bibliografii
- Rights:
- http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public