Mitochondrial cytochrome b sequences have been identified in Miniopterus schreibersii (Kuhl, 1817) from the Oriental-Australasian areas. All phylogenetic analyses indicate that the Oriental-Australasian M. schreibersii diverged from the Spanish M. schreibersii, with the mean percentage sequence differences ranging from 15.81% to 18.92% between them. A large and significant percentage sequence difference (10.91%) also separated the Chinese/Japanese specimens from the Australian specimens. Our molecular results corroborate a previous report based on morphological characters by M a e d a (1982), which suggested that Miniopterus schreibersii in Europe, Asia and Australia should be regarded as three distinct species, named Miniopterus schreibersii, M. fuliginosus and M. oceanensis. However, the specimen from Hainan should be grouped together with the other Chinese specimens in one species. The results also confirmed Appleton’s recent molecular study on Oriental-Australasian Miniopterus schreibersii.