The hematological and biochemical parameters were determined in blood of chemically immobilized pregnant and non-pregnant free ranging red, Cervus elaphus, and fallow, Dama dama, deer. In both species a marked reduction of red blood cell count, hemoglobin concentration and hematocrit, as well as higher concentration of triacylgliceride, cholesterol, creatinine and alanine aminotransferase activity were detected in pregnant animals. Significant differences in blood parameters were determined between the two cervid species. The red deer hinds had higher hemoglobin, hematocrit, mean cell volume, mean cell hemoglobin value, higher count of neutrophils and lymphocytes and a total white blood cells count, glucose, albumin, triacylgliceride and urea concentration, whereas fallow deer does had higher alanine aminotransferase activity and cholesterol concentration. The marked differences in blood glucose values were noted between pregnant animals regarding the species. Pregnant hinds had lower lymphocyte count than non-pregnant ones, while pregnant does had more than twice lymphocyte count in their blood than non-pregnant animals. Moreover, pregnant fallow deer does had rather high eosinophile count in the blood. The results provided by this research may facilitate better assessment of health status in free ranging red and fallow deer.