The potential role of intestinal intraepithelial lymphocytes (i-IELs) in the generation of host protective immunity after helminth infection was investigated using the Trichinella spiralis (Owen, 1835)/mouse model. In this study we found a significant rise of TCRyô i-IELs (P < 0.001) concurrent with the jejunal goblet cells (GC) hyperplasia in T. spiralis-infected C57BL mice on day 4 p.i. However, no direct relationship between the kinetics of the increase in TCRy5+ i-IELs and T, spiralis expulsion was observed in infected mice. Taken together, these results implicate that γδ i-IELs probably perform a unique functions related to the regulation of the CiC proliferation accompanying T. spiralis gut infection. As is known, these TCRyS* i-IELs may release mediators or growth factors that in turn influence GC differentiation. With the use of dexamethason (DEX), a potent anti-inflammatory agent which also induces apoplotic ceil death in i-IELs, wc have confirmed that the expulsion of T. spiralis from the mouse gut is accompanied by an inflammatory response. Indeed, the GC are clearly involved in these phenomena, apparently under the regulation by TCRy8+ i-lEL-mediated responses, since DEX abrogated GC proliferation in T. spiralis-infected C57BL mice and subsequently augmented adult worm burden. Our data also show that the rejection of adult worms starts concurrently with a significant increase in TCRaß* and CDS* i-IELs (P < 0.05 and P < 0.01, respectively), namely by day 7 p.i. At the same time, CD4* cells significantly decreased (P < 0.05) in the intestinal epithelium of T. spira/ir-infected, ví uninfected mice. These results may indicate that the TCRaß4 and CDS* i-IELs act as effectors of anti-7’, spiralis defence reactions. The implications of these findings for the potential role of intestinal intraepithélial CD8 and TCRaß' cells in the pathogenesis of the intestinal lesions during T. spiralis gut infection are discussed.
Time series of the daily total precipitation, daily wastewater discharges and daily concentrations and pollution loads of BOD5, COD, SS, N-NH4, Ntot and Ptot were analyzed at the inflow to the wastewater treatment plant (WWTP) for a larger city in 2004-2009 (WWTP is loaded by pollution from 435,000 equivalent inhabitants). The time series of the outflow from a WWTP was also available for 2007. The time series of daily total precipitation, daily wastewater discharges, concentrations and pollution loads at the inflow and outflow from the WWTP were standardized year by year to exclude a long-term trend, and periodic components with a period of 7 days and 365 days (and potentially also 186.5 days) were excluded from the standardized series. However, these two operations eliminated only a small part of the variance; there was a substantial reduction in the variance only for ammonium nitrogen and total nitrogen at the inflow and outflow from a WWTP. The relationship between the inflow into a WWTP and the outflow from a WWTP for the concentrations and pollution loads was described by simple transfer functions (SISO models) and more complicated transfer functions (MISO models). A simple transfer function (SISO model) was employed to describe the relationship between the daily total precipitation and the wastewater discharge.
The protein pattern of Trichuris chilensis obtained by sodium dodecyl sulfatc-polyacrylamide gel electrophoresis was analyzed. Complex protein band patterns covering a wide range of molecular weights were obtained. The molecular weights of the major proteins present in different tissue homogenatcs were estimated. Antisera raised in rabbits against homogenates of T. chilensis and sera from naturally infected Ctenomys australis were used in Western blotting, Immunoelectrophoresis and passive hemagglutination to compare the antigenicity of the adult male, adult female, eggs, oocytes, stichosome and cuticle of this parasite. Specific antibodies to parasite antigens were also detected in faecal preparations and caecum mucosal extracts of C. australis naturally infected with T, chilensis.