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Start Over Coverage 158-162

2. Humoral intestinal immunity against Encephalitozoon cuniculi (Microsporidia) infection in mice

Creator:
Sak, Bohumil and Ditrich, Oleg
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
microsporidiosis, Encephalitozoon cuniculi, intestinal immunity, and humoral antibodies
Language:
English
Description:
Three strains of mice, BALB/c, IL-12 knock-out (KO) and INF-γ knock-out, were chosen as an experimental model for the study of intestinal immunity induction against Encephalitozoon cuniculi Levaditi, Nicolau et Schoen, 1923 infection. Mice were infected perorally with 107 spores and re-infected with the same dose 70 days after the first infection. The anti-E. cuniculi IgA, IgG and IgM responses in sera and extracts of stool samples were determined by ELISA. Results have shown specific antibody production in the sera and intestinal secretions of all three strains of mice induced orally by E. cuniculi spores. BALB/c mice developed a stronger humoral immune response than IL-12 KO mice. The lowest antibody response developed in INF-γ KO mice that succumbed to the infection within 28 days post infection.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

4. Measurement of urea in the saliva of healthy mice - a pilot study

Creator:
Kovalčíková, Alexandra Gaál, Ivašková, N., Hodosy, J., Podracká, Ľ., Celec, P., and Tóthová, Ľ.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
non-invasive sampling, salivary biomarkers, reference range, experimental animals, renal failure, and technical variability
Language:
English
Description:
Salivary urea is studied as a non-invasive alternative for screening and monitoring of renal diseases. Its high variability prevents a wider clinical use. Animal experiments are needed to identify factors affecting this marker. The aim of this study was to describe the inter-individual variability of salivary urea in healthy mice, establish reference intervals, and analyse the effects of sex, age and body weight. Plasma and saliva samples were obtained from 37 male and 41 female healthy adult CD1 mice aged 13–69 weeks (body weight 22–51 g). The reference interval for salivary urea in heathy mice based on our results is 2.7–8.4 mmol/l (CV = 23 %). Multivariate analysis did not show any significant effect of age, sex, or body weight. In addition, salivary urea did not correlate with its plasma concentrations. The high variability of the promising salivary marker of kidney function in healthy mice requires further research before its use to diagnose or monitor renal failure in animal models of kidney diseases. Other potential confounders should be analysed, including intra-individual and pre-analytical variability. In addition, a normalization factor such as total salivary proteins or salivation rate is likely needed.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

7. Unreached 90th birthday of Milan Otáhal

Creator:
Tůma, Oldřich and Mareš, Jiří
Format:
bez média and svazek
Type:
model:article and TEXT
Language:
English
Description:
Milan Otáhal (1928-2017) was a leading historian studying the contemporary history of Czechoslovakia. In the 1960s, he was the head of the Department of Modern History of the Historical Institute of the Czechoslovak Academy of Sciences; in the early 1970s, he lost his job at the institute and was expelled from the Communist Party of Czechoslovakia. He was one of the fi rst signatories of Charter 77 and was active in the historical samizdat as an independent historian. Since the 1990s, his scientifi c activity was connected with the newly established Institute for Contemporary History. His main focus was the history of the anti-regime opposition and of the society between 1969 and 1989, and the role of students and intelligentsia in the change of the political situation in the end of the 1980s. He wrote a number of factographically rich and interpretationally distinctive publications on these topics. The author of the obituary mentions principal contributions of Milan Otáhal to the knowledge and understanding of Czechoslovakiaʼs most recent history, emphasizing that he was a historian who was not only intellectually refl ecting the period he was living in, but who was also intensively experiencing and co-creating it. and Přeložil: Jiří Mareš
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

8. Využití open-source hardwaru pro stavbu laboratorních přístrojů na příkladu magnetometru

Creator:
Stanke, Ladislav and Javůrek, Dalibor
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
Arduino, Hall-effect sensor, magnetometer, teslameter, Hallova sonda, magnetometr, and teslametr
Language:
Czech
Description:
In a last few years we were witnessing a raise of popularity among open-source hardware solutions, especially in the field of single board computers and microcontroller units. In these two categories several popular products can be mentioned: Arduino, Raspberry Pi, Beaglebone and Intel Edison. Currently an increase of professional or even scientific applications of these electronic devices can be observed as well. For example the Raspberry Pi computer has been used even on board of the International Space Station. The aim of this paper is to show the use of the Arduino microcontroller unit to built a device for the magnetic field measurement - a magnetometer or teslameter. To build this device a commercial sensor integrated on a chip - Continuous-Time Ratiometric Linear Hall-Effect Sensor is used. One of the paper’s goals is to demonstrate the minimization of the funding needed to build such a device, ideally without any loss of the instrument’s sensitivity or precision. Assembled instrument is compared with a purchased commercial solution made by WUNTRONIC, a KOSHAVA 5 magnetometer. and V posledních letech stoupá popularita open-source hardwaru, zejména pak jednodeskových, popřípadě jednočipových počítačů (single board computer, microcontroller unit). Do těchto dvou kategorií se řadí platformy Arduino, Raspberry Pi, Beaglebone, Intel Edison a další. V současnosti se začínají prosazovat profesionální a vědecké aplikace, které takových elektronických komponent využívají. Např. Raspberry Pi se dostalo i na palubu Mezinárodní kosmické stanice. Předložený článek si klade za cíl demonstrovat využití jednočipového počítače Arduino na stavbě magnetometru s pomocí integrovaného obvodu s Hallovou sondou. Je rovněž ukázáno, že zařízení, i přes své nízké pořizovací náklady, si zachovává užitnou hodnotu srovnatelnou s magnetometrem WUNTRONIC KOSHAVA 5 a je vhodné pro laboratorní experimenty i fyzikálně zaměřenou výuku.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public