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Start Over Coverage 150-157

2. Optimalizace oběžného kola provzdušňovacího zařízení kapalin

Creator:
Melichar , Jan
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
axial impeller wheel, aeration, agitation, treatment process, axiální oběžné kolo, provzdušňování, and míšení
Language:
Slovak
Description:
The paper deals with a topic of current interest - the problems of blending and simultaneous aeration of liquids. Modeling research making use of a number of blending propellers of different geometry was conducted towards improving the parameters of a dedicated aeration facility which employed an axial impeller - specifically, a propeller having 50 mm in diameter. The energy related parameters of blending were examined experimentally and were confronted with patterns obtained by visualizing the process of agitation of the liquid being aerated. Based on this model research, an optimal option of the 50 mm propeller was established for production purposes. Subsequently, a corresponding propeller having 80 mm in diameter was designed and manufactured based on a confirmed affinity relation of the propeller input power. A laboratory testing facility was used to carry out both qualitative and quantitative comparisons of two alternative designs of the 80 mm propeller, and the basic data required for the selection of a suitable type of propeller were established. and Příspěvek se týká aktuální problematiky míšení a současného provzdušňování kapalin. Pro zlepšení parametrů konkrétního typu provzdušňovacího zařízení s axiálním oběžným kolem - propelerem průměru 50 mm byl proveden modelový výzkum řady mísicích propelerů různého geometrického uspořádání. Experimentálně byly sledovány energetické parametry mísení, které byly konfrontovány se zviditelněnými obrazy s vizualizací procesu míšení provzdušňované kapaliny. Na základě modelového výzkumu byla pro výrobu stanovena optimální varianta propeleru o průměru 50 mm. Na základě ověřeného afinního vztahu příkonu propeleru byl navržen a vyroben odpovídající propeler o průměru 80 mm. Na laboratorním zkušebním zařízení bylo provedeno kvalitativní i kvantitativní porovnání dvou variant propelerů s průměrem 80 mm a byly získány základní údaje pro volbu vhodného typu propeleru pro aerační zařízení.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public

3. YBX1 protects against apoptosis induced by oxygen-glucose deprivation/reoxygenation in PC12 Cells via activation of the AKT/GSK3β pathway

Creator:
Tuerxun, T., Li, Xiaopeng, Hou, F., Wang, Y., Wang, X., and Ma, L.
Format:
bez média and svazek
Type:
model:article and TEXT
Subject:
YBX1, AKT/GSK3β pathway, ischaemic stroke, cerebral ischaemia, and reperfusion injury
Language:
English
Description:
Reperfusion therapies for ischaemic stroke can induce secondary injury accompanied by neuronal death. The Y-box binding protein 1 (YBX1), an oncoprotein, is critical for regulating tumour cell proliferation and apoptosis. Thus, we wanted to know whether YBX1 could regulate neuronal cell apoptosis caused by cerebral ischaemia/reperfusion (I/R). We established a model of cerebral I/R-induced injury in vitro by oxygen-glucose deprivation/reoxygenation (OGD/R) treatment and determined YBX1 expression using Western blot. Next, the effect of YBX1 on the apoptosis and viability of OGD/R-treated PC12 cells was evaluated by flow cytometry, MTT assay, and Western blot. Besides, the release of lactate dehydrogenase (LDH) and the activity of catalase (CAT) and superoxide dismutase (SOD) were detected to evaluate oxidative stress of PC12 cells induced by OGD/R. The regulatory roles of YBX1 in the AKT/GSK3β pathway were examined by Western blot. As a result, OGD/R treatment down-regulated YBX1 expression in PC12 cells. YBX1 over-expression attenuated the growth inhibition and apoptosis of PC12 cells induced by OGD/R. Besides, the increase of LDH release and the decrease of SOD and CAT activities caused by OGD/R were reversed by YBX1 over-expression. Moreover, YBX1 over-expression could activate the AKT/GSK3β pathway in OGD/ R-treated PC12 cells. Therefore, YBX1 could protect against OGD/R-induced injury in PC12 cells through activating the AKT/GSK3β signalling pathway, and thus YBX1 has the potential to become a therapeutic target for cerebral I/R-induced injury.
Rights:
http://creativecommons.org/licenses/by-nc-sa/4.0/ and policy:public