Attachment of human endothelial cells to polyester vascular grafts: pre-coating with adhesive protein assemblies and resistance to short-term shear stress

Title:

Attachment of human endothelial cells to polyester vascular grafts: pre-coating with adhesive protein assemblies and resistance to short-term shear stress

Creator:

Jaroslav Chlupáč, Eva Filová, Riedel, T., Houska, M., Eduard Brynda, Remy-Zolghadri, M., Bareille, R., Fernandez, P., Daculsi, R., Bourget, C., Bordenave, L., and Lucie Bačáková

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fb5cd16e-23da-435e-8746-01e203e4fa46
uuid:fb5cd16e-23da-435e-8746-01e203e4fa46

Subject:

Fyziologie člověka a srovnávací fyziologie, krevní cévy, polyethylentereftalát, blood-vessels, polyethylene terephthalate, blood vessel prosthesis, endothelial cells, fibrin, shear stress, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Cardiovascular prosthetic bypass grafts do not endothelialize spontaneously in humans, and so they pose a thrombotic risk. Seeding with cells improves thei r performance, particularly in small-caliber applications. Knitted tubular polyethylene- terephthalate (PET) vascular pros theses (6 mm) with commercial type I collagen (PET/Co) were modified in the lumen by the adsorption of laminin (LM), by coating with a fibrin network (Fb) or a combination of Fb and fibronectin (Fb/FN). Primary human saphenous vein endothelial cells were seeded (1.50 × 10 5 /cm 2 ), cultured for 72 h and exposed to laminar shear stress 15 dyn/cm 2 for 40 and 120 min. The control static grafts were excluded from shearing. The cell adherence after 4 h on PET/Co, PET/Co +LM, PET/Co +Fb and PET/Co +Fb/ FN was 22 %, 30 %, 19 % and 27 % of seeding, respectively. Comp ared to the static grafts, the cell density on PET/Co and PET/Co +LM dropped to 61 % and 50 %, respectively, after 120 min of flow. The cells on PET/Co +Fb and PET/Co +Fb/FN did not show any detachment during 2 h of shear stress. Pre-coating the clinically-used PET/Co vascular prosthesis with LM or Fb/FN adhesive protein assemblies promotes the adherence of endothelium. Cell retention under flow is improved particularly on fibrin-containing (Fb and Fb/FN) surfaces., J. Chlupáč ... [et al.]., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2014 Volume:63 | Number:2

Harvested from:

CDK

Metadata only:

false