Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction

Title:

Cytokine profile of human septic shock serum inducing cardiomyocyte contractile dysfunction

Creator:

Joulin, O., Petillot, P., Labalette, M., Lancel, S., and Neviere, Remi

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:fb5ccd2d-0cdc-4b34-ab2f-457cec245d98
uuid:fb5ccd2d-0cdc-4b34-ab2f-457cec245d98
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, nekróza, stárnutí buněk, human physiology, necrosis, cell aging, heart dysfunction, tumor necrosis factor, interleukin, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

This study was designed to measure nitrite/nitrate and cytokine levels of serum obtained from septic shock patients and to describe potential depressant effects of human septic serum on rat cardiomyocytes. Serum was prepared from 10 non-septic patients and 10 patients with documented septic shock. Adult rat ventricular myocytes were exposed to 20 % serum in the medium. Cardiomyocyte contractility was assessed by measuring shortening fraction and shortening velocity. Serum levels of nitrite/nitrate, a marker of nitric oxide final metabolites, and cytokines (tumor necrosis factor (TNF)-α, interleukin (IL) 1β , 6, 10, 8 and 12p70) were measured. Compared with serum from non-septic patients, serum of septic shock patients induced rapid reduction of the extent and velocity of shortening in isolated cardiomyocytes. Nitrite/nitrate, TNF-α , IL-1β and IL-12p70 concentrations of tested serum for cardiomyocyte studies were not increased in septic serum compared with controls . In contrast, septic serum that induced a depression of in vitro contractility, had increased levels of IL-6, IL-8 and IL-10. We can conclude that the depression of in vitro contractility induced by septic serum is not directly dependent on elevated levels of nitric oxide metabolites, TNF-α or IL-1β. Our results support the view that other cytokines, including IL-6, IL-8 and IL-10, are potent circulating mediators of myocardial depression in cardiomyocytes., O. Joulin, P. Petillot, M. Labalette, S. Lancel, R. Neviere., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:3

Harvested from:

CDK

Metadata only:

false