Changes in lung surfactant proteins in rats with lipololysaccharide-induced fever

Title:

Changes in lung surfactant proteins in rats with lipololysaccharide-induced fever

Creator:

Kolomaznik, M., Zila, I., Kopincova, J., Daniela Mokrá, and Andrea Čalkovská

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:f3145a6e-9a39-4c95-8747-38254a714077
uuid:f3145a6e-9a39-4c95-8747-38254a714077
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, horečka, bronchoalveolární laváž, fever, bronchoalveolar lavage, surfactant specific proteins, lipopolysaccharide, rat model, lung homogenate, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The study was designed to prove the hypothesis that lipopolysaccharide (LPS)-induced fever elicits the changes in surfactant specific proteins, potentially related to thermal tachypnea. In adult rats fever was induced by intraperitoneal administration of LPS at a dose 100 μg/kg of body weight; control group received saline. Respiratory parameters, arterial blood gases and pH and colonic body temperature (BT) were recorded. Five hours later, surfactant proteins (SP) A, B, C and D were evaluated in bronchoalveolar lavage fluid (BALF) and lung tissue (LT). LPS evoked monophasic thermic response (at 300 min 38.7±0.2 vs. 36.4±0.3 °C, P<0.05) and an increase in minute ventilation due to changes in breathing rate and tidal volume. LPS-instilled animals had higher levels of SP-A and SP-D in LT (P<0.05 and 0.01), and higher SP-D in BALF (P<0.01) than controls. SP-B increased in LT and SP-C in BALF of animals with LPS (both P0.05 vs. controls). The changes in all surfactant specific proteins are present in LPS-induced fever. Alterations of proteins related to local immune mechanisms (SP-A, SP-D) are probably a part of general inflammatory response to pyrogen. Changes in proteins related to surface activity (SP-B and SP-C) might reflect the effort of the body to stabilize the lungs in thermal challenge., M. Kolomaznik, I. Zila, J. Kopincova, D. Mokra, A. Calkovska., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2014 Volume:63 | Number:Suppl 4

Harvested from:

CDK

Metadata only:

false