Cardiac connexin-43 and PC signaling in rats with altered thyroid status without and with omega-3 fatty acids intake

Title:

Cardiac connexin-43 and PC signaling in rats with altered thyroid status without and with omega-3 fatty acids intake

Creator:

Szeiffová Bačová, B., Egan Beňová, T., Viczenczová, C., Tomáš Soukup, Hana Rauchová, Pavelka, S., Knezl, V., Miroslav Barančík, and Narcisa Tribulová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ed6ae8ab-4824-452d-8cd1-854611586551
uuid:ed6ae8ab-4824-452d-8cd1-854611586551
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, hormony štítné žlázy, srdeční arytmie, omega-3 mastné kyseliny, thyroid hormones, cardiac arrhythmia, omega-3 fatty acids, connexin-43, omega-3 polyunsaturated fatty acids, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Thyroid hormones are powerful modulators of heart function and susceptibility to arrhythmias via both genomic and non-genomic actions. We aimed to explore expression of electrical coupling protein connexin-43 (Cx43) in the heart of rats with altered thyroid status and impact of omega-3 polyunsaturated fatty acids (omega-3) supplementation. Adult male Lewis rats were divided into following six groups: euthyroid controls, hyperthyroid (treated with T3) and hypothyroid (treated with methimazol) with or without six-weeks lasting supplementation with omega-3 (20 mg/100 g/day). Left and right ventricles, septum and atria were used for immunoblotting of Cx43 and protein kinase C (PKC). Total expression of Cx43 and its phosphorylated forms were significantly increased in all heart regions of hypothyroid rats compared to euthyroid controls. In contrast, the total levels of Cx43 and its functional phosphorylated forms were decreased in atria and left ventricle of hyperthyroid rats. In parallel, the expression of PKC epsilon that phosphorylates Cx43, at serine 368, was increased in hypothyroid but decreased in hyperthyroid rat hearts. Omega-3 intake did not significantly affect either Cx43 or PKC epsilon alterations. In conclusion, there is an inverse relationship between expression of cardiac Cx43 and the levels of circulating thyroid hormones. It appears that increased propensity of hyperthyroid while decreased of hypothyroid individuals to malignant arrhythmias may be in part attributed to the changes in myocardial Cx43., B. Szeiffová Bačová, T. Egan Beňová, C. Viczenczová, T. Soukup, H. Rauchová, S. Pavelka, V. Knezl, M. Barančík, N. Tribulová., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2016 Volume:65 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false