Effect of monosodium glutamate on apoptosis and Bcl-2/Bax protein level in rat thymocyte culture

Title:

Effect of monosodium glutamate on apoptosis and Bcl-2/Bax protein level in rat thymocyte culture

Creator:

Pavlović, Voja, Cekić, S., Kocić, G., Sokolović, D., and Živković, V.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:eb06ae19-c54b-4817-9658-33b38f42efe8
uuid:eb06ae19-c54b-4817-9658-33b38f42efe8
issn:0862-8408

Subject:

Biochemie. Molekulární biologie. Biofyzika, fyziologie živočichů, cytologie, apoptóza, animal physiology, cytology, apoptosis, glutaman sodný, tymocyty, Bcl-2, Bax, monosodium glutamate, thymocytes, 2, and 577

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Monosodium glutamate (MSG), the sodium salt of glutamate, is commonly used as a flavor enhancer in modern nutrition. Recent studies have shown th e existence of glutamate receptors on lymphocytes, thymoc ytes and thymic stromal cells. In this study, we evaluated the in vitro effect of different MSG concentr ations on rat thymocyte apoptosis and expression of two apoptosis-related proteins, Bcl-2 and Bax. Rat thymocytes, obtained from male Wistar rats, were exposed to increasing concentrations of MSG (ranging from 1 mM to 100 mM) for 24 h. Apoptosis was detected using the Annexin V-FITC/PI apoptosis detection kit and cells were analyzed using a flow cytometer. Expression of Bcl-2 and Bax proteins were determined with flow cytometry using respective monoclonal antibodies. Exposure to MSG resulted in a dose-dependent decrease in cell survival (as determined by trypan blue exclusion method). Annexin V- FITC/PI also confirmed that MSG incr eased, in a dose-dependent manner, ap optotic cell death in rat thymocyte cultures. MSG treatment induced downregulation of Bcl-2 protein, while Bax protein levels were not significantly changed. Our data showed that MSG significantly modulates thymocyte apoptosis rate in cultures. The temporal profile of Bcl-2 and Bax expression after MSG treatment suggests that downregulation of Bcl-2 protein and the resulting change of Bcl-2/Bax protein ratio may be an important event in thymocyte apoptosis triggered by MSG., V. Pavlović, S. Cekić, G. Kocić, D. Sokolović, V. Živković., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:5

Harvested from:

CDK

Metadata only:

false