PLAG1 promotes high glucose-induced angiogenesis and migration of retinal endothelial cells by regulating the Wnt/β-catenin signalling pathway

Title:

PLAG1 promotes high glucose-induced angiogenesis and migration of retinal endothelial cells by regulating the Wnt/β-catenin signalling pathway

Creator:

Gu, Q. and Wei, Haifeng

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e60025ba-7e88-4e8c-b07f-cd87f89cca63
uuid:e60025ba-7e88-4e8c-b07f-cd87f89cca63

Subject:

PLAG1, high glucose, angiogenesis, migration, retinal, endothelial cells, Wnt/β-catenin, and diabetic retinopathy

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Proliferation and migration of retinal endothelial cells (RECs) contribute to the development of diabetic retinopathy. PLAG1 (pleomorphic adenoma gene 1) functions as a zinc-finger transcription factor to participate in the development of lipoblastomas or pleomorphic adenomas of the salivary glands through regulation of cell proliferation and migration. The role of PLAG1 in diabetic retinopathy was investigated in this study. Firstly, RECs were induced under high glucose conditions, which caused reduction in viability and induction of apoptosis in the RECs. Indeed, PLAG1 was elevated in high glucosetreated RECs. Functional assays showed that silence of PLAG1 increased viability and suppressed apoptosis in high glucose-induced RECs, accompanied with up-regulation of Bcl-2 and down-regulation of Bax and cleaved caspase-3. Moreover, migration of RECs was promoted by high glucose conditions, while repressed by knockdown of PLAG1. High glucose also triggered angiogenesis of RECs through up-regulation of vascular endothelial growth factor (VEGF). However, interference of PLAG1 reduced VEGF expression to retard the angiogenesis. Silence of PLAG1 also attenuated high glucose-induced up-regulation of Wnt3a, β-catenin and c-Myc in RECs. Moreover, silence of PLAG1 ameliorated histopathological changes in the retina of STZ-induced diabetic rats through down-regulation of β-catenin. In conclusion, knockdown of PLAG1 suppressed high glucose-induced angiogenesis and migration of RECs, and attenuated diabetic retinopathy by inactivation of Wnt/ β-catenin signalling.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

25-32

Source:

Folia biologica | 2022 Volume:68 | Number:1

Harvested from:

CDK

Metadata only:

false