Silymarin component 2,3-dehydrosilybin attenuates cardiomyocyte damage following hypoxia/reoxygenation by limiting oxidative stress

Title:

Silymarin component 2,3-dehydrosilybin attenuates cardiomyocyte damage following hypoxia/reoxygenation by limiting oxidative stress

Creator:

Gabrielová, E., Vladimír Křen, Martin Jabůrek, and Martin Modrianský

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e5baf015-9f07-4029-9094-847f9a5613be
uuid:e5baf015-9f07-4029-9094-847f9a5613be
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, silymarin, dehydrosilybin, neonatal rat cardiomyocytes, postconditioning, reactive oxygen species, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Ischemic postconditioning and remote conditioning are potentially useful tools for protecting ischemic myocardium. This study tested the hypothesis that 2,3-dehydrosilybin (DHS), a flavonolignan component of Silybum marianum , could attenuate cardiomyocyte damage following hypoxia/ reoxygenation by decreasing the generation of reactive oxygen species (ROS). After 5-6 days of cell culture in normoxic conditions the rat neonatal cardiomyocytes were divided into four groups. Control group (9 h at normoxic conditions), hypoxia/ reoxygenation group (3 h at 1 % O2 , 94 % N2 and 5 % CO2 followed by 10 min of 10 μmol·l -1 DHS and 6 h of reoxygenation in normoxia) and postconditioning group (3 h of hypoxia, three cycles of 5 min reoxygenation and 5 min hypoxia followed by 6 h of normoxia). Cell viability assess ed by propidium iodide staining was decreased after DHS treatment consistent with increased levels of lactatedehydrogenase (LDH) after reoxygenation. LDH leakage was significantly reduced when cardiomyocytes in the H/Re group were exposed to DHS. DHS treatment reduced H2O2 production and also decreased the generation of ROS in the H/Re group as evidenced by a fluorescence indicator. DHS treatment reduces reoxygenation-induced injury in cardiomyocytes by attenuation of ROS generation, H2O2 and protein carbonyls levels. In addition, we found that both the postconditioning protocol and the DHS treatment are associated with restored ratio of phosphorylated/total protein kinase C epsilon, relative to the H/Re group. In conclusion, our data support the protective role of DH S in hypoxia/reperfusion injury and indicate that DHS may act as a postconditioning mimic., E. Gabrielová, V. Křen, M. Jabůrek, M. Modrianský., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:1

Harvested from:

CDK

Metadata only:

false