Study on the regulation mechanism of lipopolysaccharide on oxidative stress and lipid metabolism of bovine mammary epithelial cells

Title:

Study on the regulation mechanism of lipopolysaccharide on oxidative stress and lipid metabolism of bovine mammary epithelial cells

Creator:

Li, Lin, Tang, Weibin, Zhao, Mei, Gong, Binbin, Cao, Meng, and Li, Jianyuan

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:e4410c15-52be-4170-ba3c-820365388f51
uuid:e4410c15-52be-4170-ba3c-820365388f51
issn:1802-9973
doi:10.33549/physiolres.934682

Subject:

oxidační stres, mastné kyseliny, oxidative stress, fatty acids, lipopolysaccharide, MAC-T, oxidative stress damage, triglyceride, fatty acid biosynthesis, 14, and 612

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

a1_The long-term feeding of a high-concentrate diet (the concentrate ratio is greater than 60 %) leads to mammary gland inflammatory response in ruminants and decreased quality in dairy cows and affects the robust development of the dairy industry. The main reason is closely related to elevated lipopolysaccharide (LPS) in the body. In this experiment, a bovine mammary epithelial cell line (MAC-T) was used as a model, and LPS at different concentrations (0 ng/ml, 1 ng/ml, 10 ng/ml, 100 ng/ml, 1000 ng/ml, 10000 ng/ml) was added to the cells. The cell survival rate, oxidative stress indicators, total lipid droplet area, triglyceride content and key genes regulating lipid metabolism were detected by 3-(4,5)-dimethylthiahiazo(-z-y1)- 3,5-di-phenytetrazoliumromide (MTT), assay kit, microscope observation and RT-PCR methods to explore the regulatory mechanism of mammary health and milk fat synthesis.The results showed that compared with those of the control group, the survival rates of cells were significantly decreased after 9 h of stimulation with 1000 ng/ml and 10000 ng/ml LPS (P<0.01).The contents of superoxide dismutase (SOD), catalase (CAT) and total antioxidant capacity (T-AOC) in cells were significantly decreased (P<0.05).Compared with that of the control group, the content of malondialdehyde (MDA) in cells was significantly increased (P<0.05) after stimulation with 10000 ng/ml LPS for 9 h. After 9 h of stimulation with 100 ng/ml, 1000 ng/ml and 10000 ng/ml LPS, the total lipid drop area and triglyceride (TG) content of MAC-T cells were significantly decreased (P<0.05).The expression levels of fatty acid synthesis-related genes AcetylCoA carboxylase (ACC) and Stearoyl-CoA desaturase 1 (SCD-1) were significantly decreased after 9 h of stimulation with 100 ng/ml, 1000 ng/ml and 10000 ng/ml LPS (P<0.05), while the expression levels of Fatty Acid synthetase (FAS) were significantly decreased after stimulation with 1000 ng/ml and 1000 ng/ml LPS (P<0.05)., Lin Li, Weibin Tang, Mei Zhao, Binbin Gong, Meng Cao, Jianyuan Li., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

777-785

Source:

Physiological research | 2021 Volume:70 | Number:5

Harvested from:

CDK

Metadata only:

false