Cardioprotection induced by remote ischemic preconditioning preserves the mitochondrial respiratory function in acute diabetic myocardium

Title:

Cardioprotection induced by remote ischemic preconditioning preserves the mitochondrial respiratory function in acute diabetic myocardium

Creator:

Kancirová, I., Jašová, M., Muráriková, M., Sumbalová, Z., Uličná, O., Ravingerová, T., Waczulíková, I., Ziegelhöffer, A., and Ferko, M.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:dc26b505-5679-4dba-ba61-2593fb596e3e
uuid:dc26b505-5679-4dba-ba61-2593fb596e3e

Subject:

Myocardial preconditioning, Mitochondria, Energy metabolism, Mitochondrial A TP synthase, and Myocardial ischemic reperfusion injury

Type:

article, model:article, and TEXT

Description:

A 2×2 factorial design was used to evaluate possible preservation of mitochondrial functions in two cardioprotective experimental models, remote ischemic preconditioning and streptozotocin-induced diabetes mellitus, and their interaction during ischemia/reperfusion injury (I/R) of the heart. Male Wistar rats were randomly allocated into four groups: control (C), streptozotocin-induced diabetic (DM), preconditioned (RPC) and preconditioned streptozotocin-induced diabetic (DM+RPC). RPC was conducted by 3 cycles of 5-min hind-limb ischemia and 5-min reperfusion. DM was induced by a single dose of 65mg/kg streptozotocin. Isolated hearts were exposed to ischemia/reperfusion test according to Langendorff. Thereafter mitochondria were isolated and the mitochondrial respiration was measured. Additionally, the ATP synthase activity measurements on the same preparations were done. Animals of all groups subjected to I/Rexhibited a decreased state 3 respiration with the least change noted in DM+RPC group associated with no significant changes in state 2 respiration. In RPC, DM and DM+RPC group, no significant changes in the activity of ATP synthase were observed after I/R injury. These results suggest that the endogenous protective mechanisms of RPC and DM do preserve the mitochondrial function in heart when they act in combination.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2016 Volume:65 | Number:Suppl 5

Harvested from:

CDK

Metadata only:

false