Atropa Belladonna L. water extract: modulator of extracellular matrix formation in vitro and in vivo

Title:

Atropa Belladonna L. water extract: modulator of extracellular matrix formation in vitro and in vivo

Creator:

Petr Gál, Vasilenko, T., Kováč, I., Kostelníková, M., Jakubčo, J., Pavol Szabo, Barbora Dvořánková, František Sabol, Hans-Joachim Gabius, and Karel Smetana

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:ce8d0cb9-b4a0-4128-bcf5-f64fc78447a4
uuid:ce8d0cb9-b4a0-4128-bcf5-f64fc78447a4

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, phytotherapy, aqueous extract, wound healing, fibroblast, keratinocyte, inflammation, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Previously, we found that treatment of cutaneous wounds with Atropa belladonna L. (AB) revealed shortened process of acute inflammation as well as increased tensile strength and collagen deposition in healing skin wounds (Gál et al. 2009). To better understand AB effect on skin wound healing male SpragueDawley rats were submitted to one round full thickness skin wound on the back. In two experimental groups two different concentrations of AB extract were daily applied whereas the control group remained untreated. For histological evaluation samples were removed on day 21 after surgery and stained for wide spectrum cytokeratin, collagen III, fibronectin, galectin-1, and vimentin. In addition, in the in vitro study different concentration of AB extract were used to evaluate differences in HaCaT keratinocytes proliferation and differentiation by detection of Ki67 and keratin-19 expressions. Furthermore, to assess ECM formation of human dermal fibroblasts on the in vitro level fibronectin and galectin-1 were visualized. Our study showed that AB induces fibronectin and galectin-1 rich ECM formation in vitro and in vivo. In addition, the proliferation of keratinocytes was also increased. In conclusion, AB is an effective modulator of skin wound healing. Nevertheless, further research is needed to find optimal therapeutic concentration and exact underlying mechanism of action., P. Gál ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:3

Harvested from:

CDK

Metadata only:

false