Protochlorophyllide photo-transformation and chlorophyll(ide) formation in barley etioplast fractions

Title:

Protochlorophyllide photo-transformation and chlorophyll(ide) formation in barley etioplast fractions

Creator:

Kovacevic, D., Dewez, D., and Popovic, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c7638ca9-269d-4a4d-b9b1-342d7643b448
uuid:c7638ca9-269d-4a4d-b9b1-342d7643b448
issn:0300-3604
doi:10.1007/s11099-007-0058-5

Subject:

fluorescence emission spectra, Hordeum, and NADPH:protochlorophyllide oxidoreductase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Localization of protochlorophyll(ide) (Pchlide) forms and chlorophyllide (Chlide) transformation process were studied by using comparative analyses of de-convoluted 77 K fluorescence spectra of barley etioplast stroma and different membrane fractions obtained by sucrose gradient centrifugation. Non-photoactive 633 nm Pchlide form was mainly located in the envelope-prothylakoid membrane mixture while the photoactive 657 nm Pchlide was dominant pigment in the prolamellar body membrane and in the soluble etioplast fraction (stroma). When these fractions were exposed to a saturating flash, conversion of photoactive Pchlide into 697 nm Chlide was preferential in the prolamellar body and in the stroma, while the 676 nm Chlide was dominant pigment form in the envelope-prothylakoid fraction. These spectral characteristics are considered to reflect molecular composition and organization of the pigment-protein complexes specific for each etioplast compartment. and D. Kovacevic, D. Dewez, R. Popovic.

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

340-347

Source:

Photosynthetica | 2007 Volume:45 | Number:3

Harvested from:

CDK

Metadata only:

false