Effects of nitrogen deficiency on gas exchange, chlorophyll fluorescence, and antioxidant enzymes in leaves of rice plants

Title:

Effects of nitrogen deficiency on gas exchange, chlorophyll fluorescence, and antioxidant enzymes in leaves of rice plants

Creator:

Huang, Z.-A., Jiang, D.-A., Yang, Y., Sun, J.-W., and Jin, S.-H.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:c69cdccc-e707-4870-ac11-2a81780c2087
uuid:c69cdccc-e707-4870-ac11-2a81780c2087
issn:0300-3604
doi:10.1023/B:PHOT.0000046153.08935.4c

Subject:

catalase, leaf senescence, malonyldialdehyde, Oryza sativa, peroxidase, photosynthesis, photosystem 2, ribulose-1,5-bisphosphate carboxylase/oxygenase, stomatal conductance, and transpiration rate

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Gas exchange, chlorophyll (Chl) fluorescence, and contents of photosynthetic pigments, soluble proteins (ribulose-1,5-bisphosphate carboxylase/oxygenase, RuBPCO), and antioxidant enzymes were characterized in the fully expanded 6th leaves in rice seedlings grown on either complete (CK) or on nitrogen-deficient nutrient (N-deficiency) solutions during a 20-chase period. Compared with the control plants, the lower photosynthetic capacity at saturation irradiance (Pmax) was accompanied by an increase in intercellular CO2 concentration (Ci), indicating that in N-deficient plants the decline in Pmax was not due to stomatal limitation but due to the reduced carboxylation efficiency. The fluorescence parameters ΦPS2, Fv'/Fm', electron transport rate (ETR), and qP showed the same tendency as Pmax in N-deficient plants. Correspondingly, a higher qN paralleled the rise of the ratio of carotenoid (Car) to Chl contents. However, Fv/Fm was still diminished, suggesting that photoinhibition did occur in the photosystem 2 (PS2) reaction centres. In addition, the activities of antioxidant enzymes on a fresh mass basis were gradually lowered, leading to the aggravation of membrane lipid peroxidation with the proceeding N-deficiency. The accumulation of malonyldialdehyde resulted in the lessening of Chl and soluble protein content. Analyses of regression showed PS2 excitation pressure (1 - qP) was linearly correlated with the content of Chl and inversely with soluble protein (particularly RuBPCO) content. There was a lag phase in the increase of PS2 excitation pressure compared to the decrease of RuBPCO content. Therefore, the increased excitation pressure under N-deficiency is probably the result of saturation of the electron transport chain due to the limitation of the use of reductants by the Calvin cycle. Rice plants responded to N-deficiency and high irradiance by decreasing light-harvesting capacity and by increasing thermal dissipation of absorbed energy. and Z.-A. Huang ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

357-364

Source:

Photosynthetica | 2004 Volume:42 | Number:3

Harvested from:

CDK

Metadata only:

false