Cell stratification, spheroid formation and bioscaffolds used to grow cells in three dimensional cultures

Title:

Cell stratification, spheroid formation and bioscaffolds used to grow cells in three dimensional cultures
Cell stratification, spheroid formation and bioscaffolds used to grow cells in three dimensional cultures

Creator:

Hrebíková, Hana, Čížková, Dana, Chvátalová, Jana, Pisal, Rishikaysh, Adamčik, Richard, Beznoska, Pavel, Díaz-Garcia, Daniel, and Mokrý, Jaroslav

Contributor:

Hrebíková, Hana, Čížková, Dana , 1977-, Chvátalová, Jana, Pisal, Rishikaysh, Adamčik, Richard, Beznoska, Pavel, Díaz-Garcia, Daniel, and Mokrý, Jaroslav , 1964-

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc16011073-b7a1f01a-6012-4992-9bfc-86326d22262b
uuid:bmc16011073-b7a1f01a-6012-4992-9bfc-86326d22262b
local:bmc16011073
http://actamedica.lfhk.cuni.cz/
doi: 10.14712/18059694.2015.97
local: bmc16011073

Subject:

zvířata, buněčné kultury, buněčné linie, proliferace buněk--fyziologie, hepatocyty--fyziologie, lidé, myši, myoblasty, sféroidy buněčné--fyziologie, tkáňové inženýrství--metody, and tkáňové podpůrné struktury

Type:

model:article, article, Text, časopisecké články, práce podpořená grantem, and TEXT

Format:

print, text, and regular print

Description:

The cell culture became an invaluable tool for studying cell behaviour, development, function, gene expression, toxicity of compounds and efficacy of novel drugs. Although most results were obtained from cell cultivation in two-dimensional (2D) systems, in which cells are grown in a monolayer, three-dimensional (3D) cultures are more promising as they correspond closely to the native arrangement of cells in living tissues. In our study, we focused on three types of 3D in vitro systems used for cultivation of one cell type. Cell morphology, their spatial distribution inside of resulting multicellular structures and changes in time were analysed with histological examination of samples harvested at different time periods. In multilayered cultures of WRL 68 hepatocytes grown on semipermeable membranes and non-passaged neurospheres generated by proliferation of neural progenitor cells, the cells were tightly apposed, showed features of cell differentiation but also cell death that was observable in short-term cultures. Biogenic scaffolds composed of extracellular matrix of the murine tibial anterior muscle were colonized with C2C12 myoblasts in vitro. The recellularized scaffolds did not reach high cell densities comparable with the former systems but supported well cell anchorage and migration without any signs of cell regression. and H. Hrebíková, D. Čížková, J. Chvátalová, R. Pisal, R. Adamčik, P. Beznoska, D. Díaz-Garcia, J. Mokrý

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

Acta medica (Hradec Králové) Universitas Carolina, Facultas Medica Hradec Králové--MED00010947

Source:

Acta medica (Hradec Králové) | 2015 Volume:58 | Number:3

Harvested from:

CDK

Metadata only:

false

Date:

2015