Effects of MnTnHex-2-PyP on markers of inflammation and lipid peroxidation in asthma mice model

Title:

Effects of MnTnHex-2-PyP on markers of inflammation and lipid peroxidation in asthma mice model
Effects of MnTnHex-2-PyP on markers of inflammation and lipid peroxidation in asthma mice model

Creator:

Veneta Shopova, Lyudmil Terziev, Violeta Dancheva, Galya Stavreva, Milena Atanasova, Angelina Stoyanova, and Tzvetan Lukanov

Contributor:

Veneta Shopova, Lyudmil Terziev, Violeta Dancheva, Galya Stavreva, Milena Atanasova, Angelina Stoyanova, and Tzvetan Lukanov

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bmc14045963-8f50cd49-8fe9-456d-80ce-5fd79442c471
uuid:bmc14045963-8f50cd49-8fe9-456d-80ce-5fd79442c471
local:bmc14045963
http://ojs.journals.cz/index.php/EMHPJ/issue/archive
local: bmc14045963

Subject:

myši inbrední C57BL, modely nemocí na zvířatech, biologické markery, zánět, bronchiální astma--metabolismus, peroxidace lipidů, ovalbumin, hypertonické roztoky, bronchoalveolární lavážní tekutina, oxidační stres, časové faktory, zvířata, ženské pohlaví, and metaloporfyriny--farmakologie--metabolismus

Type:

model:article, article, Text, statistics, and TEXT

Format:

braille, electronic resource, remote, and elektronický zdroj

Description:

Background and objective: Investigation of the effects of MnTnHex-2-PyP on some markers of inflammation and lipid peroxidation in an asthma mice model. Methods: The experiment was carried out on 24 female mice C57Bl/6, divided into four groups: group 1, controls; group 2, injected with ovalbumin (OVA); group 3, treated with MnTnHex-2-PyP and group 4, treated with OVA and MnTnHex-2-PyP. The animals from groups 1 and 3 were injected i.p. on days 0 and 14 with a 100 μl phosphate-buffered saline (PBS), and those from groups 2 and 4 were injected with a 100 μl ovalbumin solution, containing 20 μg OVA. On days 24, 25 and 26 the mice from groups 1 and 2 were inhaled with PBS for 30 min, and those from groups 2 and 4 were given a 1% ovalbumin solution. One hour before inhalation, and 12 hours later the animals from groups 1 and 2 were injected i.p. with 100 μl PBS, and those from groups 3 and 4 received a 100 μl MnTnHex-2-Pyp solution in PBS сontaining 0.05mg/kg. Results: Ovalbumin alone (group 2) increased the total cell number, total protein content, the levels of IL-4, IL-5 and 8-isoprostane in bronchoalveolar lavage. Elevations were observed in IgE level in serum, and the malone dialdehyde (MDA) content in the lung homogenate. These markers were decreased significantly in group 4 as compared to the OVA group. Conclusions: MnTnHex-2-Pyp reduces the inflammation and lipid peroxidation in Ovalbumin-induced mice asthma model, Veneta Shopova, Lyudmil Terziev, Violeta Dancheva, Galya Stavreva, Milena Atanasova, Angelina Stoyanova, Tzvetan Lukanov, and Literatura

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Relation:

European medical, health and pharmaceutical journal--MED00177703

Source:

European medical, health and pharmaceutical journal : open access reviewed journal | 2011 Volume:2011 | Number:2

Harvested from:

CDK

Metadata only:

false

Date:

2011