Stem cells regenerative properties on new rat spinal fusion model

Title:

Stem cells regenerative properties on new rat spinal fusion model

Creator:

Klíma, K., Vaněček, V., Aleš Kohout, Jiroušek, O., René Foltán, Vladimír Machoň, Gabriela Pavlíková, Pavla Jendelová, Eva Syková, and Jiří Šedý

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:bc2701f8-aa2e-45e3-8d87-3df30fab68c6
uuid:bc2701f8-aa2e-45e3-8d87-3df30fab68c6
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, animal model, rat, MSCs, bone graft substitute, spinal fusion, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Stem cells biology is one of the most frequent topic of physiological research of today. Spinal fusion represents common bone biology challenge. It is the indicator of osteoinduction and new bone formation on ectopic model. The purpose of this study was to establish a simple model of spinal fusion based on a rat model including verification of the possible use of titanium microplates with hydroxyapatite scaffold combined with human bone marrow-derived mesenchymal stem cells (MSCs). Spinous processes of two adjacent vertebrae were fixed in 15 Wistar rats. The space between bony vertebral arches and spinous processes was either filled with augmentation material only and covered with a resorbable collagen membrane (Group 1), or filled with augmentation material loaded with 5 × 10 6 MSCs and covered with a resorbable collagen membrane (Group 2). The rats were sacrificed 8 weeks after the surgery. Histology, histomorphometry and micro-CT were performed. The new model of interspinous fusion was safe, easy, inexpensive, with zero mortality. We did not detect any substantial pathological changes or tumor formation after graft implantation. We observed a nonsignificant effect on the formation of new bone tissue between Group 1 and Group 2. In the group with MSCs (Group 2) we described mino r inflamatory response which indicates the imunomodulational and antiinflamatory role of MSCs. In conclusion, this new model proved to be easy to use in small animals like rats., K. Klíma, V. Vaněček, A. Kohout, O. Jiroušek, R. Foltán, J. Štulík, V. Machoň, G. Pavlíková, P. Jendelová, E. Syková, J. Šedý., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:1

Harvested from:

CDK

Metadata only:

false