Antioxidative effect of dietary flavonoid isoquercitrin on human ovarian granulosa cells HGL5 in vitro

Title:

Antioxidative effect of dietary flavonoid isoquercitrin on human ovarian granulosa cells HGL5 in vitro

Creator:

Kolesárová, Adriana, Michalcová, Katarína, Roychoudhury, Shubhadeep, Baldovská, Simona, Tvrdá, Eva, Vašíček, Jaromír, Chrenek, Peter, Sanislo, Ľuboslav, and Křen, Vladimír

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b2b76d65-8799-479d-b248-740528bfb3f5
uuid:b2b76d65-8799-479d-b248-740528bfb3f5
issn:1802-9973
doi:10.33549/physiolres.934692

Subject:

flavonoidy, antioxidanty, steroidní hormony, flavonoids, antioxidants, steroid hormons, granulosa cells, transforming growth factor, 14, and 612

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

This study aimed to examine the effect of dietary flavonoid isoquercitrin on ovarian granulosa cells using the immortalized human cell line HGL5. Cell viability, survival, apoptosis, release of steroid hormones 17β-estradiol and progesterone, and human transforming growth factor-β2 (TGF-β2) and TGF-β2 receptor as well as intracellular reactive oxygen species (ROS) generation were investigated after isoquercitrin treatment at the concentration range of 5-100 μg.ml-1 . It did not cause any significant change (p>0.05) in cell viability as studied by AlamarBlue assay in comparison to control. No significant change was observed (p>0.05) in the proportion of live, dead and apoptotic cells as revealed by apoptotic assay using flow cytometry. Similarly, the release of 17β-estradiol, progesterone, TGF-β2 and its receptor were not affected significantly (p>0.05) by isoquercitrin as detected by ELISA, in comparison to control. Except for the highest concentration of 100 μg.ml-1 , which led to oxidative stress, isoquercitrin exhibited antioxidative activity at lower concentration used in the study (5, 10, 25, and 50 μg.ml-1 ) by hampering the production of intracellular ROS, in comparison to control, as detected by chemiluminescence assay (p<0.05). Findings of the present study indicate an existence of the antioxidative pathway that involves inhibition of intracellular ROS generation by isoquercitrin in human ovarian granulosa cells., Adriana Kolesárová, Katarína Michalcová, Shubhadeep Roychoudhury, Simona Baldovská, Eva Tvrdá, Jaromír Vašíček, Peter Chrenek, Ľuboslav Sanisló, Vladimír Křen., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

745-754

Source:

Physiological research | 2021 Volume:70 | Number:5

Harvested from:

CDK

Metadata only:

false