Impact of chemoprophylaxis immunisation under halofantrine (CPS-HF) drug cover in Plasmodium yoelii Swiss mice malaria model

Title:

Impact of chemoprophylaxis immunisation under halofantrine (CPS-HF) drug cover in Plasmodium yoelii Swiss mice malaria model

Creator:

Siddiqui, Arif Jamal, Bhardwaj, Jyoti, Hamadou, Walid Sabri, Goyal, Manish, Jahan, Sadaf, Ashraf, Syed Amir, Jamal, Arshad, Sharma, Pankaj, Sachidanandan, Manojkumar, Badraoui, Riadh, Snoussi, Mejdi, and Adnan, Mohd

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:b10e87a5-65ff-421c-9936-8cb3109e25e3
uuid:b10e87a5-65ff-421c-9936-8cb3109e25e3
doi:10.14411/fp.2022.003

Subject:

CPS-immunisation, sporozoites, cytokines, Real Time PCR, and mRNA expression

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

In the present study, we have investigated the role of antimalarial drug halofantrine (HF) in inducing the sterile protection against challenges with sporozoites of the live infectious Plasmodium yoelii (Killick-Kendrick, 1967) in Swiss mice malaria model. We observed that during the first to third sequential sporozoite inoculation cycles, blood-stage patency remains the same in the control and chemoprophylaxis under HF drug cover (CPS-HF) groups. However, a delayed blood-stage infection was observed during the fourth and fifth sporozoite challenges and complete sterile protection was produced following the sixth sporozoite challenge in CPS-HF mice. We also noticed a steady decline in liver stage parasite load after 3th to 6th sporozoite challenge cycle in CPS-HF mice. CPS-HF immunisation results in a significant up-regulation of pro-inflammatory cytokines (IFN-γ, TNF-α, IL-12 and iNOS) and down-regulation of anti-inflammatory cytokines (IL-10 and TGF-β) mRNA expression in hepatic mononuclear cells (HMNC) and spleen cells in the immunised CPS-HF mice (after 6th sporozoite challenge) compared to control. Overall, our study suggests that the repetitive sporozoite inoculation under HF drug treatment develops a strong immune response that confers protection against subsequent challenges with sporozoites of P. yoelii.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

1-10

Source:

Folia parasitologica | 2022 Volume:69 | Number:1

Harvested from:

CDK

Metadata only:

false