Transgenic tomato plants overexpressing chloroplastic monodehydroascorbate reductase are resistant to salt- and PEG-induced osmotic stress

Title:

Transgenic tomato plants overexpressing chloroplastic monodehydroascorbate reductase are resistant to salt- and PEG-induced osmotic stress

Creator:

Li, F., Wu, Q. Y., Duan, M., Dong, X. C., Li, B., and Meng, Q. W.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:acf9dfca-46f7-4d70-a3c2-88b24ce9c16b
uuid:acf9dfca-46f7-4d70-a3c2-88b24ce9c16b
issn:0300-3604
doi:10.1007/s11099-012-0021-y

Subject:

botanika, botany, ascorbate, monodehydroascorbate reductase, osmotic stresses, reactive oxygen species, tomato, 2, and 58

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

RNA gel hybridization showed that the expression of monodehydroascorbate reductase (MDHAR) in the wild type (WT) tomato was decreased firstly and then increased under salt- and polyethylene glycol (PEG)-induced osmotic stress, and the maximum level was observed after treatment for 12 h. WT, sense transgenic and antisense transgenic tomato plants were used to analyze the antioxidative ability to cope with osmotic stresses. After salt stress, the fresh mass (FM) and height of sense transgenic lines were greater than those of antisense lines and WT plants. Under salt and PEG treatments, sense transgenic plants showed a lower level of hydrogen peroxide (H2O2) and malondialdehyde (MDA), a higher net photosynthetic rate (PN), and the maximal photochemical efficiency of PSII (Fv/Fm) compared with WT and antisense transgenic plants. Moreover, sense lines maintained higher ascorbate peroxidase (APX) activity than WT and antisense plants under salt- and PEG-induced osmotic stress. These results indicate that chloroplastic MDHAR plays an important role in alleviating photoinhibition of PSII by elevating ascorbate (AsA) level under salt- and PEG-induced osmotic stress., F. Li ... [et al.]., and Obsahuje bibliografii

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

120-128

Source:

Photosynthetica | 2012 Volume:50 | Number:1

Harvested from:

CDK

Metadata only:

false