The effect of heme oxygenase on ganglioside redistribution within hepatocytes in experimental estrogen-induced cholestasis

Title:

The effect of heme oxygenase on ganglioside redistribution within hepatocytes in experimental estrogen-induced cholestasis

Creator:

Tomáš Petr, Šmíd, V., Kučerová, V., Váňová, K., Martin Leníček, Libor Vítek, and Šmíd, F.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a872c068-1d44-4f48-8cc9-47215a033a1d
uuid:a872c068-1d44-4f48-8cc9-47215a033a1d
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, oxidační stres, oxidative stress, GM1 ganglioside, heme oxygenase, intrahepatic cholestasis, 17α-ethinylestradiol, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Cholestasis is characterized by the elevation of serum total bile acids (TBA), which leads to the production of both free radicals and oxidative stress. Although they do not share the same mechanisms, membrane glycosphingolipids (GSL) and the antioxidant enzyme heme oxygenase-1 (HMOX1) both act against the pro-oxidative effect of TBA. The aim of the study was to assess the role of HMOX on GSL redistribution and composition within hepatocytes in the rat model of estrogen-induced cholestasis. Compared to the controls, an increase of total gangliosides in the liver homogenates of the cholestatic group (P=0.001) was detected; further, it paralleled along with the activation of their biosynthetic b-branch pathway (P<0.01). These effects were partially prevented by HMOX activation. Cholestasis was accompanied by a redistribution of GM1 ganglioside from the cytoplasm to the sinusoids; while HMOX activation led to the retention of GM1 in the cytoplasm (P=0.014). Our study shows that estrogen-induced cholestasis is followed by changes in the synthesis and/or distribution of GSL. These changes are not only triggered by the detergent power of accumulated TBA, but also by their pro-oxidant action. Increases in the antioxidant defenses might represent an important supportive therapeutic measure for patients with cholestatic liver disease., T. Petr, V. Šmíd, V. Kučerová, K. Váňová, M. Leníček, L. Vítek, F. Šmíd, L. Muchová., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2014 Volume:63 | Number:3

Harvested from:

CDK

Metadata only:

false