In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells

Title:

In vitro study of reactive oxygen species production during photodynamic therapy in ultrasound-pretreated cancer cells

Creator:

Hana Kolářová, Bajgar, R., Kateřina Tománková, Krestýn, E., Doležal, L., and Jan Hálek

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a67cb758-e435-4b7c-bdd6-1752cbc2625f
uuid:a67cb758-e435-4b7c-bdd6-1752cbc2625f
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie, physiology, photodynamic therapy, sonodynamic therapy, phthalocyanine sensitizer, reactive oxygen species, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Several recent studies bring evidence of cell death enhancement in photodynamic compound loaded cells by ultrasonic treatment. There are a number of hypotheses suggesting the mechanism of the harmful ultrasonic effect. One of them considers a process in the activation of photosensitizers by ultrasonic energy. Because the basis of the photodynamic damaging effect on cells consists in the production of reactive oxygen species (ROS), we focused our study on whether the ultrasound can increase ROS production within cancer cells. Particularly, we studied ROS formation in ultrasound pretreated breast adenocarcinoma cells during photodynamic therapy in the presence of chloroaluminum phthalocyanine disulfonate (ClAlPcS2). Production of ROS was investigated by the molecular probe CM-H2DCFDA. Our results show that ClAlPcS2 induces higher ROS production in the ultrasound pretreated cell lines at a concentration of 100 μM and light intensity of 2 mW/cm2. We also observed a dependence of ROS production on photosensitizer concentration and light dose. These results demonstrate that the photodynamic effect on breast cancer cells can be enhanced by ultrasound pretreatment., H. Kolářová, R. Bajgar, K. Tománková, E. Krestýn, L. Doležal, J. Hálek., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2007 Volume:56 | Number:Suppl 1

Harvested from:

CDK

Metadata only:

false