Proteomic analysis of potential immunoreactive proteins from muscle larvae and adult worms of Trichinella spiralis in experimentally infected pigs

Title:

Proteomic analysis of potential immunoreactive proteins from muscle larvae and adult worms of Trichinella spiralis in experimentally infected pigs

Creator:

Bien, Justyna, Cabaj, Wladyslaw, and Bożena Moskwa

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:a1e65d50-30ed-4422-9921-6389ad2353ec
uuid:a1e65d50-30ed-4422-9921-6389ad2353ec
issn:1803-6465
doi:10.14411/fp.2015.022

Subject:

antigeny, hmotnostní spektrometrie, antigens, mass spectroscopy, Trichinella, 2, and 59

Type:

model:article and TEXT

Format:

electronic, počítač, and online zdroj

Description:

The present study was undertaken to identify potentially immunoreactive proteins of the muscle larvae (ML) and adult stage (Ad) of the nematode Trichinella spiralis Owen, 1835. To identify immunoreactive proteins that are specifically recognised by anti-Trichinella antibodies, ML and Ad crude extracts and their excretory-secretory (E-S) products were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot with serum samples from pigs experimentally infected with T. spiralis. A total of 18 bands were selected for final identification by liquid chromatography-tandem mass spectrometry. To further understand the functions of the proteins identified in this study, gene ontology terms were applied. Results showed that the specific antibodies against T. spiralis reacted with protein bands matching heat shock proteins, aminopeptidase, enolase, isocitrate dehydrogenase NADP-dependent, tropomyosin, P49 antigen, serine proteinase, secreted 5'-nucleotidase, antigen targeted by protective antibodies, 53 kDa E-S antigen, putative trypsin and paramyosin. Three proteins common for both adult stage and muscle larvae, including heat shock proteins, enolase and 5'-nucleotidase, might play important role during T. spiralis infection. These proteins are presumably presented to the host immune system and may induce humoral immune response. Thus, these proteins may be potential antigens for early diagnosis and the development of a vaccine against the parasite., Justyna Bien, Wladyslaw Cabaj, Bozena Moskwa., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

1-8

Source:

Folia parasitologica | 2015 Volume:62 | Number:1

Harvested from:

CDK

Metadata only:

false