Early changes during skin repair using tissue-engineered dermal template in a full-thickness burn

Title:

Early changes during skin repair using tissue-engineered dermal template in a full-thickness burn

Creator:

Gál, P., Fröhlichová, L., Čoma, M., Pafčuga, I., Šuca, H., Grossová, I., Hríbiková, Z., Sticová, E., and Zajíček, R.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:9283fba3-baab-410c-87f0-9827b99a7d2e
uuid:9283fba3-baab-410c-87f0-9827b99a7d2e

Subject:

wound healing, angiogenesis, artificial skin, tissue engineering, and burn injury

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Rapid wound closure in extensively burned patients has remained one of the major unresolved issues of medicine. Integra® is the most widely established artificial skin, which is composed of a porous matrix of cross-linked bovine collagen and chondroitin 6-sulphate covered by a semi-permeable silicone layer. We present here a (immuno)histological study of a severely burned patient with a full-thickness burn treated with a tissue-engineered dermal template (Integra®) and split-thickness skin graft-based protocol. Immunohistochemical investigation of the artificial dermis revealed that immune cell infiltration reached its peak on day 10. Tissue immunophenotyping found an increase in CD3+ cells over the course of the study as well as CD4 and CD8 positivity on day 40, indicating remaining T-cell subpopulations. We observed weak/no infiltration of NK cells (CD56+). In conclusion, the use of bi-layer Integra® represents a feasible and safe procedure resulting in formation of non-irritating dermal substitutes.

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Coverage:

161-168

Source:

Folia biologica | 2020 Volume:66 | Number:5-6

Harvested from:

CDK

Metadata only:

false