Comparison of two approaches to measurement of electrical impedance of glass microelectrodes designed for evaluation of temperature changes in biological tissues

Title:

Comparison of two approaches to measurement of electrical impedance of glass microelectrodes designed for evaluation of temperature changes in biological tissues

Creator:

Rech, F., Dittert, I., and Vyskočil, F.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:82d01f87-f84b-465c-83d4-f556d79f7db5
uuid:82d01f87-f84b-465c-83d4-f556d79f7db5

Subject:

microelectrode, temperature measurement, and electrical impedance measurement

Type:

article, model:article, and TEXT

Description:

We proposed a temperature sensitive microelectrode for rapid measurements of temperature at the cellular level. In principle, the electrical impedance of the tip of the microelectrode changes with temperature. We designed an impulse measurement system (STEP) sensitive to the above changes of impedance. The system is based on a presettable negative input impedance of the current to a voltage converter. We compared the efficiency of the new STEP with the currently used RAMP system. We found following advantages of the STEP system: i) the danger of high voltage oscillations which could mechanically destroy the microelectrode tip is eliminated; ii) this system provides the opportunity to set the maximum sensitivity of the system according to the measured temperature interval. Moreover, the STEP method makes it possible to measure the resistance by using a sinusoidal stimulation signal which has to be preliminarily compensated by a rectangular signal. The shortest sampling period of the new system represents 0.1 ms with a resolution higher than 0.1 K and sensitivity better than 30 mV/K.

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 1992 Volume:41 | Number:3

Harvested from:

CDK

Metadata only:

false