Genistein does not inhibit TGF-β1-induced conversion of human dermal fibroblasts to myofibroblasts

Title:

Genistein does not inhibit TGF-β1-induced conversion of human dermal fibroblasts to myofibroblasts

Creator:

Kaňuchová, Miriam, Urban, Lukáš, Melegová, Nikola, Čoma, Matúš, Dvořánková, Barbora, Smetana, Karel, and Gál, Peter

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7ab27996-df2d-4334-8c93-62b015b75854
uuid:7ab27996-df2d-4334-8c93-62b015b75854
issn:1802-9973
doi:10.33549/physiolres.934666

Subject:

hojení ran, fytoestrogeny, wound healing, phytoestrogens, selective estrogen receptor modulator, 14, and 612

Type:

model:article and TEXT

Format:

počítač and online zdroj

Description:

Transforming growth factor beta 1 (TGF-β1) is a pro-fibrotic cytokine with a key role in wound repair and regeneration, including induction of fibroblast-to-myofibroblast transition. Genistein is a naturally occurring selective estrogen receptor modulator with promising anti-fibrotic properties. In the present study we aimed to investigate whether genistein modulates TGF-β1 (canonical and non-canonical) signaling in normal dermal fibroblasts at the protein level (Western blot and immunofluorescence). We demonstrated that TGF-β1 induces the myofibroblast-like phenotype in the studied fibroblast signaling via canonical (SMAD) and non-canonical (AKT, ERK1/2, ROCK) pathways. Genistein induced only ERK1/2 expression, whereas the combination of TGF-β1 and genistein attenuated the ERK1/2 and ROCK signaling. Of note, the other studied pathways remained almost unaffected. From this point of view, genistein does not impair conversion of normal fibroblasts to myofibroblast-like cells., Miriam Kaňuchová, Lukáš Urban, Nikola Melegová, Matúš Čoma, Barbora Dvořánková, Karel Smetana Jr., Peter Gál., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

815-820

Source:

Physiological research | 2021 Volume:70 | Number:5

Harvested from:

CDK

Metadata only:

false