Experimental and theoretical study on high temperature induced changes in chlorophyll a fluorescence oscillations in barley leaves upon 2 % CO2

Title:

Experimental and theoretical study on high temperature induced changes in chlorophyll a fluorescence oscillations in barley leaves upon 2 % CO2

Creator:

Lazár, D., Kaňa , R., Klinkovský, T., and Nauš, J.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:7a740435-15be-4033-a062-81a2301418eb
uuid:7a740435-15be-4033-a062-81a2301418eb
issn:0300-3604
doi:10.1007/s11099-005-3027-x

Subject:

Hordeum vulgare, model, NADPH, and 1,5-bisphosphate carboxylase, oxygenase and its activase

Type:

model:article and TEXT

Format:

bez média and svazek

Description:

Oscillations in many of photosynthetic quantities with a period of about 1 min can be routinely measured with higher plant leaves after perturbation of the steady state by sudden change in gas phase. Among all hypotheses suggested so far to explain the oscillations, an effect of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) activation status to control the oscillations is highly probable, at least upon high temperature (HT) treatment when in vivo RuBPCO activity controlled by RuBPCO activase (RuBPCO-A) decreases. Therefore, we measured the oscillations in fluorescence signal coming from barley leaves (Hordeum vulgare L. cv. Akcent) after their exposure for various time intervals to different HTs in darkness. We also evaluated steady state fluorescence and CO2 exchange parameters to have an insight to functions of electron transport chain within thylakoid membrane and Calvin cycle before initiation of the oscillations. The changes in period of the oscillations induced by moderate HT (up to 43 °C) best correlated with changes in non-photochemical fluorescence quenching (qN) that in turn correlated with changes in gross photosynthetic rate (PG) and rate of RuBPCO activation (kact). Therefore, we suggest that changes in period of the oscillations caused by moderate HT are mainly controlled by RuBPCO activation status. For more severe HT (45 °C), the oscillations disappeared which was probably caused by an insufficient formation of NADPH by electron transport chain within thylakoid membrane as judged from a decrease in photochemical fluorescence quenching (qP). Suggestions made on the basis of experimental data were verified by theoretical simulations of the oscillations based on a model of Calvin cycle and by means of a control analysis of the model. and D. Lazár ... [et al.].

Language:

Multiple languages

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Coverage:

13-27

Source:

Photosynthetica | 2005 Volume:43 | Number:1

Harvested from:

CDK

Metadata only:

false