Expression of estrogen receptor α and β in rat astrocytes in primary culture: effects of hypoxia and glucose deprivation

Title:

Expression of estrogen receptor α and β in rat astrocytes in primary culture: effects of hypoxia and glucose deprivation

Creator:

Al-Bader, M. D., Malatiali, S. A., and Redzic, Z. B.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:763dbeb8-c083-4068-a0bb-55561adafa2b
uuid:763dbeb8-c083-4068-a0bb-55561adafa2b

Subject:

Fyziologie člověka a srovnávací fyziologie, hypoxie, ischemie, mozek, hypoxia, ischemia, brain, astrocytes, estrogen receptor, glucose deprivation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print

Description:

Estrogen replacement therapy could play a role in the reduction of injury associated with cerebral ischemia in vivo, which could be, at least partially, a consequence of estrogen influence of glutamate buffering by astrocytes during hypoxia/ischemia. Estrogen exerts biological effects through interaction with its two receptors: estrogen receptor alpha (ERα) and estrogen receptor beta (ERβ), which are both expressed in astrocytes. This study explored effects of hypoxia and glucose deprivation (HGD), alone or followed by 1 h recovery, on ERα and ERβ expression in primary rat astrocyte cultures following 1 h exposure to: a) 5 % CO2 in air (control group-CG); b) 2 % O2/5 % CO2 in N2 with glucose deprivation (HGD group-HGDG); or c) the HGDG protocol followed by 1 h CG protocol (recovery group-RG). ER α mRNA expression decreased in HGDG. At the protein level, full-length ER α (67 kDa) and three ER α -immunoreactive protein bands (63, 60 and 52 kDa) were detected. A significant decrease in the 52 kDa band was seen in HGDG, wh ile a significant decrease in expression of the full length ERα was seen in the RG. ERβ mRNA and protein expression (a 54 kDa single band) did not change. The observed decrease in ER α protein may limit estrogen-mediated signalling in astrocytes during hypoxia and recovery., M. D. Al-Bader, S. A. Malatiali, Z. B. Redzic., and Obsahuje bibliografii a bibliografické odkazy

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2011 Volume:60 | Number:6

Harvested from:

CDK

Metadata only:

false