Macrophage phenotypes in the adipose tissue of postmenopausal women

Title:

Macrophage phenotypes in the adipose tissue of postmenopausal women

Creator:

Králová, A., Ivana K. Lesná, Jiří Froněk, Čejková, S., Sekerková, A., Libor Janoušek, Thieme, F., Ilja Stříž, Jana Ždychová, and Rudolf Poledne

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:707736ed-a97e-44d6-8ad6-7189b680a0f2
uuid:707736ed-a97e-44d6-8ad6-7189b680a0f2
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, menopauza, tuková tkáň, záněty, menopause, adipose tissue, inflammations, macrophages, calprotectin, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Atherosclerosis pathology is the interplay between high intrav ascular LDL particle concentration and monocyte/ macrophage presence within the sub -endothelial space of the artery. In this project, phenotypes of macrophages connected with subclinical inflammation in adipose tissue of living kidney donors were studied. Samples of subcutaneous adipose tissue of living kidney donors (n=36) were exposed to collagenase. Stromal vascular fraction (SVF) was eluted from the samples, then labeled with monoclonal antibodies (anti- CD14 and anti -calprotectin), conjugated with fluo rochromes and analy zed by flow cytometry. The positive correlation between the number of total macrophages and calprotectin- positive macrophages with BMI in the subcutaneous adipose tissue of postmenopausal women was demonstrated (p<0.05; R=0.43 and p<0.01 ; R=0.60), whereas no positive correlation in premenopausal women and men was shown. In conclusion, we documented a significant effect of BMI increase on the presence of total macrophages in adipose tissue of postmenopausal women, in contrast to premenopausal women. This difference was much more pronounced when proinflammatory macrophages with membrane- bound calprotectin were analyzed., A. Králová, I. Králová Lesná, J. Froněk, S. Čejková, A. Sekerková, L. Janoušek, F. Thieme, I. Stříž, J. Ždychová, R. Poledne., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:Suppl 3

Harvested from:

CDK

Metadata only:

false