Age-associated changes in Ca2+-ATPase and oxidative damage in sarcoplasmic reticulum of rat heart

Title:

Age-associated changes in Ca2+-ATPase and oxidative damage in sarcoplasmic reticulum of rat heart

Creator:

Eva Babušíková, Ján Lehotský, Dušan Dobrota, Račay, P., and Peter Kaplán

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:6e101794-16a7-4e5c-8aea-572538ee129f
uuid:6e101794-16a7-4e5c-8aea-572538ee129f

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, aging, Ca2+-ATPase, protein damage, heart, oxidative stress, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Altered Ca2+ handling may be responsible for the development of cardiac contractile dysfunctions with advanced age. In the present study, we investigated the roles of oxidative damage to sarcoplasmic reticulum (SR) and expression of Ca2+-ATPase (SERCA 2a) and phospholamban in age-associated dysfunction of cardiac SR. SR vesicles were prepared from hearts of 2-, 6-, 15-, and 26-month-old Wistar rats. Although activity of Ca2+-ATPase decreased with advancing age, no differences in relative amounts of SERCA 2a and phospholamban protein were observed. On the other hand, significant accumulation of protein oxidative damage occurred with aging. The results of this study suggest that agerelated alteration in Ca2+-ATPase activity in the rat heart is not a consequence of decreased protein levels of SERCA 2a and phospholamban, but could arise from oxidative modifications of SR proteins. Cellular oxidative damage caused by reactive oxygen species could contribute to age-related alternations in myocardial relaxation., E. Babušíková ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:5

Harvested from:

CDK

Metadata only:

false