Exercise-induced muscle damage impairs insulin signaling pathway associated with IRS-1 oxidative modification

Title:

Exercise-induced muscle damage impairs insulin signaling pathway associated with IRS-1 oxidative modification

Creator:

Aoi, W., Naito, Y., Tokuda, H., Tanimura, Y., Oya-Ito, T., and Yoshikawa, T.

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:63c46714-1bce-4e4c-bd1e-0b9d9c21c024
uuid:63c46714-1bce-4e4c-bd1e-0b9d9c21c024

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, muscle damage, glucose uptake, oxidative stress, 4-HNE, IRS-1, 14, and 612

Type:

article, články, model:article, and TEXT

Description:

Strenuous exercise induces delayed-onset muscle damage including oxidative damage of cellular components. Oxidative stress to muscle cells impairs glucose uptake via disturbance of insulin signaling pathway. We investigated glucose uptake and insulin signaling in relation to oxidative protein modification in muscle after acute strenuous exercise. ICR mice were divided into sedentary and exercise groups. Mice in the exercise group performed downhill running exercise at 30 m/min for 30 min. At 24 hr after exercise, metabolic performance and insulin-signaling proteins in muscle tissues were examined. In whole body indirect calorimetry, carbohydrate utilization was decreased in the exercised mice along with reduction of the respiratory exchange ratio compared to the rested control mice. Insulin-stimulated uptake of 2-deoxy-[3 H]glucose in damaged muscle was decreased after acute exercise. Tyrosine phosphorylation of insulin receptor substrate (IRS)-1 and phosphatidyl-3-kinase/Akt signaling were impaired by exercise, leading to inhibition of the membrane translocation of glucose transporter 4. We also found that acute exercise caused 4-hydroxy-nonenal modification of IRS-1 along with elevation of oxidative stress in muscle tissue. Impairment of insulin-induced glucose uptake into damaged muscle after strenuous exercise would be related to disturbance of insulin signal transduction by oxidative modification of IRS-1., W. Aoi ... [et al.]., and Obsahuje seznam literatury

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2012 Volume:61 | Number:1

Harvested from:

CDK

Metadata only:

false