On the pharmacology of oxidative burst of human neutrophils

Title:

On the pharmacology of oxidative burst of human neutrophils

Creator:

Radomír Nosáľ, Katarína Drábiková, Viera Jančinová, Tatiana Mačičková, Jana Pečivová, Perečko, T., Juraj Harmatha, and Jan Šmidrkal

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5edae2d5-13ad-4520-ad44-7a7259b7794e
uuid:5edae2d5-13ad-4520-ad44-7a7259b7794e
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, proteinové kinázy, apoptóza, protein kinases, apoptosis, human neutrophils, oxidative burst, chemiluminescence, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The effect of three therapeutically used drugs and five polyphenolic compounds on the mechanism of oxidative burst was compared in whole blood and isolated neutrophils at cellular and molecular level. In 10 μM concentration, the compounds investigated decreased the oxidative burst of whole blood in the rank order of potency: N-feruloylserotonin (N-f-5HT) > curcumin (CUR) > quercetin (QUER) > arbutin (ARB) > resveratrol (RES) > dithiaden (DIT) > carvedilol (CARV) > brompheniramine (BPA). The ratio between the percentage inhibition of extracellular versus intracellular chemiluminescence (CL) followed the rank order QUER > N-f-5HT > RES > CUR > DIT and is indicative of the positive effect of the compounds tested against oxidative burst of neutrophils, demonstrating suppression of reactive oxygen species extracellularly with minimal alteration of intracellular reactive oxygen species (ROS). Activation of protein kinase C was significantly decreased by DIT, CUR, QUER and N-f-5HT. CARV, DIT, QUER and ARB reduced activated neutrophil myeloperoxidase release more significantly compared with the effect on superoxide anion generation. All compounds tested increased the activity of caspase-3 in cell-free system. It is suggested that other regulatory mechanisms than protein kinase C might participate in the inhibition of neutrophil activation with the compounds tested. Different mechanisms are concerned in controlling the assembly of NADPH oxidase and the regulatory role of calcium ions is suggested. Compounds decreasing the amount of extracellular ROS generation, yet affecting but minimally intracellular ROS generation, are promising for further investigation in vivo., R. Nosáľ, K. Drábiková, V. Jančinová, T. Mačičková, J. Pečivová, T. Perečko, J. Harmatha, J. Šmidrkal., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/publicdomain/mark/1.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:Suppl 4

Harvested from:

CDK

Metadata only:

false