Changes of Sodium and ATP Affinities of Renal Na,K-ATPase During and After Nitric Oxide-Deficient Hypertension

Title:

Changes of Sodium and ATP Affinities of Renal Na,K-ATPase During and After Nitric Oxide-Deficient Hypertension

Creator:

Vrbjar, N., Veronika Javorková, and Oľga Pecháňová

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:5b0d2e9b-f48b-4d5f-9bf9-c88a142d40c4
uuid:5b0d2e9b-f48b-4d5f-9bf9-c88a142d40c4
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, fyziologie člověka, human physiology, Sodium pump, Nitric oxide, Hypertension, L-NAME, Kidney, 14, and 612

Type:

article, studie, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

The aim of this study was to assess the molecular basis of renal Na,K-ATPase disturbances in response to NO-deficient hypertension induced in rats by NO-synthase inhibition with 40 mg/kg/day NG-nitro-L-arginine methyl ester (L-NAME) for four weeks. After 4-week administration of L-NAME, the systolic blood pressure (SBP) increased by 30 %. Three weeks after terminating the treatment, SBP recovered to control value. When activating the Na,K-ATPase with its substrate ATP, a 36 % increase in Km and 29 % decrease in Vmax values were observed in NO-deficient rats. During activation with Na+, the Vmax was decreased by 20 % and the KNa was increased by 111 %, indicating a profound decrease in the affinity of the Na+-binding site in NO-deficient rats. After spontaneous recovery from hypertension, the Vmax remained at the level as in hypertension for both types of enzyme activation. However, in the presence of lower concentrations of substrate which are of physiological relevance an improvement of the enzyme activity was observed as documented by return of Km for ATP to control value. The KNa value for Na+ was decreased by 27 % as compared to hypertension, but still exceeded the corresponding value in the control group by 55 % thus resulting in a partial restoration of Na+ affinity of Na,K-ATPase which was depressed as a consequence of NO-dependent hypertension., N. Vrbjar, V. Javorková, O. Pecháňová., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2002 Volume:51 | Number:5

Harvested from:

CDK

Metadata only:

false