Hepatocyte transplantation attenuates the course of acute liver failure induced by thioacetamide in Lewis rats

Title:

Hepatocyte transplantation attenuates the course of acute liver failure induced by thioacetamide in Lewis rats

Creator:

Eva Koblihová, Lukšan, O., Mrázová, I., Ryska, M., and Luděk Červenka

Identifier:

https://cdk.lib.cas.cz/client/handle/uuid:59d523a1-94d5-4a39-9727-6df138253a7e
uuid:59d523a1-94d5-4a39-9727-6df138253a7e
issn:0862-8408

Subject:

Fyziologie člověka a srovnávací fyziologie, orgánové selhání, játra, organ failure, liver, acute liver failure, trioacetamide, hepatocyte transplantation, 14, and 612

Type:

article, články, model:article, and TEXT

Format:

print, bez média, and svazek

Description:

Acute liver failure (ALF) is a clinical syndrome resulting from widespread damage of hepatocytes, with extremely high mortality rate. Urgent orthotopic liver transplantation was shown to be the most effective therapy for ALF but this treatment option is limited by sca rcity of donor organs. Therefore, hepatocyte transplantation (Tx) has emerged as a new therapeutical measure for ALF, however, the first clinical applications proved unsatisfactory. Apparently, extensive preclinical studies are needed. Our aim was to exami ne if hepatocytes isolated from transgenic “firefly luciferase” Lewis rats into the recipient liver would attenuate the course of thioacetamide (TAA) -induced ALF in Lewis rats. Untreated Lewis rats after TAA administration showed a profound decrease in sur vival rate; no animal survived 54 h. The rats showed marked increases in plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities, in plasma level of bilirubin and ammonia (NH 3 ), and in a significant decrease in plasma albumin. Hepatocyte Tx attenuated the course of TAA -induced ALF Lewis rats which was reflected by improved survival rate and reduced degree of liver injury showing as lowering of elevated plasma ALT, AST, NH 3 and bilirubin levels and increasing plasma albumin. In addition, bioluminescence imaging analyses have shown that in the TAA- damaged livers the transplanted hepatocyte were fully viable throughout the experiment. In conclusion, the results show that hepatocyte Tx into the liver can attenuate the course of TAA- induced ALF in Lewis rats. This information should be considered in attempts to develop new therapeutic approaches to the treatment of ALF., E. Koblihová, O. Lukšan, I. Mrázová, M. Ryska, L. Červenka., and Obsahuje bibliografii

Language:

English

Rights:

http://creativecommons.org/licenses/by-nc-sa/4.0/
policy:public

Source:

Physiological research | 2015 Volume:64 | Number:5

Harvested from:

CDK

Metadata only:

false